通过监测 DNA 电荷转移诱导的荧光闪烁来检测单核苷酸变异。
Detection of single-nucleotide variations by monitoring the blinking of fluorescence induced by charge transfer in DNA.
机构信息
The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Japan.
出版信息
Chembiochem. 2013 Aug 19;14(12):1430-3. doi: 10.1002/cbic.201300380. Epub 2013 Jul 11.
PMID:23846860
Abstract
Charge transfer dynamics in DNA: Photo-induced charge separation and charge-recombination dynamics in DNA was assessed by monitoring the blinking of fluorescence. Single nucleotide variations, mismatch and one base deletion, were differentiated based on the length of the off-time of the blinking, which corresponds to the lifetime of the charge-separated state.
摘要
DNA 中的电荷转移动力学：通过监测荧光的闪烁来评估 DNA 中的光致电荷分离和电荷复合动力学。基于闪烁的关闭时间（对应于电荷分离态的寿命）的长度，可以区分单核苷酸变异、错配和一个碱基缺失。
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