通过圆盘电泳法对银屑病鳞屑中磷酸酶进行分离及分子量估计。
Separation and molecular weight estimation of phosphatases in psoriatic scales by disc-electrophoresis.
作者信息
Förster F J, Balikcioglu S, Leonhardi G
出版信息
Arch Dermatol Forsch. 1975;252(2):85-90. doi: 10.1007/BF00561831.
Disc-electrophoretic investigations of psoriatic scale homogenates (15000 x g supernatant) revealed several different phosphatase activities. At pH 5 either five different phosphatase active bands (substrates: p-nitrophenylphosphate, naphthylphosphate) or three different bands (substrate: glycerophosphate) could be obtained. At pH 7 only one band (substrate: p-nitrophenylphosphate) showed phosphatase activity. At pH 9.9 either two bands (substrate: p-nitrophenylphosphate) or three bands (substrate: glycerophosphate) could be demonstrated. the acid p-nitrophenylphosphatase activity of the psoriatic specific bands "9" and "10" could be distinguished by their different fluoride and tartrate susceptibility. Also the alkaline glycerophosphatase activities could be differentiated by distinct Ca and Mg susceptibility.
对银屑病鳞屑匀浆（15000×g 上清液）进行圆盘电泳研究，发现了几种不同的磷酸酶活性。在 pH 5 时，使用对硝基苯磷酸酯、萘基磷酸酯作为底物可得到五条不同的磷酸酶活性带，使用甘油磷酸酯作为底物则可得到三条不同的带。在 pH 7 时，只有一条带（底物：对硝基苯磷酸酯）显示出磷酸酶活性。在 pH 9.9 时，使用对硝基苯磷酸酯作为底物可显示出两条带，使用甘油磷酸酯作为底物则可显示出三条带。银屑病特异性条带“9”和“10”的酸性对硝基苯磷酸酶活性可通过它们对氟化物和酒石酸盐的不同敏感性来区分。碱性甘油磷酸酶活性也可通过对钙和镁的不同敏感性来区分。
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