莱茵衣藻磷酸酶：利用特定突变体的生物化学和细胞化学方法
Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants.
作者信息
Matagne R F, Loppes R, Deltour R
出版信息
J Bacteriol. 1976 May;126(2):937-50. doi: 10.1128/jb.126.2.937-950.1976.
The unicellular alga Chlamydomonas reinhardi produces two constitutive acid phosphatases and three depressible phosphatases (a neutral and two alkaline ones) that can utilize napthyl phosphate as a substrate. Specific mutants depressible phosphatase were used to investigate biochemical properties and the cytochemical localization of these enzymes. The two constitutive phosphatases show similar pH optima (about 5.0) and Km values (2 x 10(-3) to 3.3 x 10(-3) M) but differ in their heat sensitivity and affinity for glycerophosphate.
单细胞藻类莱茵衣藻可产生两种组成型酸性磷酸酶和三种可诱导型磷酸酶（一种中性和两种碱性磷酸酶），这些酶能够利用萘基磷酸作为底物。利用可诱导型磷酸酶的特定突变体来研究这些酶的生化特性和细胞化学定位。两种组成型磷酸酶表现出相似的最适pH值（约5.0）和Km值（2×10⁻³至3.3×10⁻³M），但它们在热敏感性和对甘油磷酸的亲和力方面存在差异。
Zotero 插件