[从阿胶炒珠酶解物中分离抗氧化肽及其质谱分析]
[Isolation and mass spectrometric analysis of antioxidant peptides from enzymatic hydrolysates of roasted pills of Asini Corii Colla].
作者信息
Li Xiao-Chen, Yan Li-Hua, Wang Zhi-Min, Zhang Qi-Wei, Gao Jian-Ping, Chen Liang-Mian, Wang Jin-Yu, Tong Yan, Zhang Gui-Feng
机构信息
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Apr;38(7):1021-5.
A fraction named GFC-1 with high antioxidant activities in vitro was isolated from the enzymatic hydrolysates of roasted pills of Asini Corii Colla, and the peptides in this fraction were identified. The enzymatic hydrolysates were isolated and purified with anion exchange chromatography and Sephadex G-25 filtration chromatography successively. GFC-1, a fraction isolated from the hydrolysates, exhibited the highest DPPH and ABTS scavenging capacity (DPPH 47. 95% at 2.0 g x L(-1) and ABTS 97.20% at 0.40 g x L(-1). Nine peptides from GFC-1 were identified by LC-ESI-MS/MS coupled with TurboSEQUEST search software and Swiss-Prot data base, and a high repetition core sequence GPAGPPGPP was also found.
从阿胶焙炒丸酶解产物中分离出一种在体外具有高抗氧化活性的组分GFC-1，并对该组分中的肽进行了鉴定。酶解产物先后通过阴离子交换色谱和Sephadex G-25过滤色谱进行分离和纯化。从水解产物中分离得到的组分GFC-1表现出最高的DPPH和ABTS清除能力（在2.0 g·L⁻¹时DPPH清除率为47.95%，在0.40 g·L⁻¹时ABTS清除率为97.20%）。通过LC-ESI-MS/MS结合TurboSEQUEST搜索软件和Swiss-Prot数据库鉴定了GFC-1中的9种肽，还发现了一个高重复核心序列GPAGPPGPP。
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