Chinchalikar A J, Aswal V K, Kohlbrecher J, Wagh A G
Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai-400085, India.
Phys Rev E Stat Nonlin Soft Matter Phys. 2013 Jun;87(6):062708. doi: 10.1103/PhysRevE.87.062708. Epub 2013 Jun 18.
The liquid-liquid phase transition (LLPT) in aqueous salt solutions of lysozyme protein has been studied by small-angle neutron scattering. Measurements have been carried out on fixed protein concentration with varying salt concentration approaching LLPT. The data are fitted considering protein interaction by the two Yukawa (2Y) potential which combines short-range attraction and long-range repulsion. We show that LLPT arises because of enhancement of non-DLVO (Derjaguin-Landau-Verwey-Overbeek) short-range attraction without any conformational structural change of the protein. The salt concentration required for LLPT as well as corresponding short-range attraction decreases significantly with increase in protein concentration.
通过小角中子散射研究了溶菌酶蛋白盐水溶液中的液-液相转变（LLPT）。在固定蛋白质浓度下，随着盐浓度接近LLPT而变化时进行了测量。考虑蛋白质通过结合短程吸引和长程排斥的双 Yukawa（2Y）势相互作用对数据进行拟合。我们表明，LLPT 的出现是由于非 DLVO（Derjaguin-Landau-Verwey-Overbeek）短程吸引力增强，而蛋白质没有任何构象结构变化。LLPT 所需的盐浓度以及相应的短程吸引力随着蛋白质浓度的增加而显著降低。
