Istituto di Biofisica, Consiglio Nazionale delle Ricerche, c/o Fondazione Bruno Kessler, Via alla Cascata 56/C, 38123 Trento, Italy; Dipartimento di Fisica, Università di Trento, Via Sommarive 14, 38050 Povo, Trento, Italy.
Biophys Chem. 2013 Dec 1;182:58-63. doi: 10.1016/j.bpc.2013.06.013. Epub 2013 Jun 27.
Cellular imaging techniques have become powerful tools in cell biology. With respect to others, the techniques based on vibrational spectroscopy present a clear advantage: the molecular composition and the modification of subcellular compartments can be obtained in label-free conditions. In fact, from the evolution of positions, intensities and line widths of Raman and infrared bands in the cell spectra, characteristic information on cellular activities can be achieved, and particularly, cellular death can be investigated. In this work we present the time evolution of the Raman spectra of single live Jurkat cells (T-lymphocyte) by looking at the high frequency part of their Raman spectra, that is the CH stretching region, around 3000cm(-1). In particular, investigation into the composition or rearrangement of CH bounds, markers of cellular membrane fatty acids, can represent an important method to study and to recognize cell death. The experimental procedure we used, together with the analysis of these high frequency vibrational bands, may represent a new, improved and advantageous approach to this kind of study.
细胞成像技术已成为细胞生物学中强有力的工具。与其他技术相比，基于振动光谱的技术具有明显的优势：可以在非标记条件下获得亚细胞区室的分子组成和修饰。事实上，从细胞光谱中拉曼和红外谱带的位置、强度和线宽的演变，可以获得有关细胞活动的特征信息，特别是可以研究细胞死亡。在这项工作中，我们通过观察其拉曼光谱的高频部分（即 CH 伸缩区域，约 3000cm(-1)），研究了单个活 Jurkat 细胞（T 淋巴细胞）的拉曼光谱的时间演变。特别是，对 CH 键的组成或重排（细胞膜脂肪酸的标志物）的研究可以成为研究和识别细胞死亡的重要方法。我们使用的实验程序以及对这些高频振动带的分析，可能代表了这种研究的一种新的、改进的和有利的方法。
