Nocchetti Morena, Piccotti Chiara, Piccinini Michela, Caponi Silvia, Mattarelli Maurizio, Pietrella Donatella, Di Michele Alessandro, Ambrogi Valeria
Dipartimento di Scienze Farmaceutiche, Università di Perugia, Via del Liceo, 1, 06123 Perugia, Italy.
Istituto Officina dei Materiali, National Research Council (IOM-CNR), Unit of Perugia, c/o Department of Physics and Geology, University of Perugia, Via A. Pascoli, 06123 Perugia, Italy.
Nanomaterials (Basel). 2024 Oct 12;14(20):1637. doi: 10.3390/nano14201637.
The need to develop synthetic bone substitutes with structures, properties, and functions similar to bone and capable of preventing microbial infections is still an ongoing challenge. This research is focused on the preparation and characterization of three-dimensional porous scaffolds based on hydroxyapatite (HA)-functionalized calcium carbonate loaded with silver nanoparticles and simvastatin (SIMV). The scaffolds were prepared using the foam replica method, with a polyurethane (PU) sponge as a template, followed by successive polymer removal and sintering. The scaffolds were then coated with poly(lactic-co-glycolic) acid (PLGA) to improve mechanical properties and structural integrity, and loaded with silver nanoparticles and SIMV. The scaffolds were characterized by X-ray powder diffraction (XRD), field emission scanning electron microscopy (FE-SEM), ATR FT-IR, and silver and SIMV loading. Moreover, the samples were analyzed by Brillouin and Raman microscopy. Finally, in vitro bioactivity, SIMV and silver release, and antimicrobial activity against and were evaluated. From the Brillouin spectra, samples showed characteristics analogous to those of bone tissue. They exhibited new hydroxyapatite growth, as evidenced by SEM, and good antimicrobial activity against the tested bacteria. In conclusion, the obtained results demonstrate the potential of the scaffolds for application in bone repair.
开发具有与骨相似的结构、性质和功能且能够预防微生物感染的合成骨替代物仍然是一项持续的挑战。本研究聚焦于基于负载银纳米颗粒和辛伐他汀（SIMV）的羟基磷灰石（HA）功能化碳酸钙的三维多孔支架的制备与表征。使用泡沫复制法制备支架，以聚氨酯（PU）海绵为模板，随后依次去除聚合物并进行烧结。然后用聚乳酸 - 乙醇酸共聚物（PLGA）对支架进行涂层以改善机械性能和结构完整性，并负载银纳米颗粒和SIMV。通过X射线粉末衍射（XRD）、场发射扫描电子显微镜（FE - SEM）、衰减全反射傅里叶变换红外光谱（ATR FT - IR）以及银和SIMV负载量对支架进行表征。此外，通过布里渊和拉曼显微镜对样品进行分析。最后，评估了体外生物活性、SIMV和银的释放以及对 和 的抗菌活性。从布里渊光谱来看，样品显示出与骨组织相似的特征。如扫描电子显微镜所示，它们呈现出新的羟基磷灰石生长，并且对测试细菌具有良好的抗菌活性。总之，所得结果证明了该支架在骨修复应用中的潜力。
