Uehara M, Oomori S, Kitagawa H, Ueshima T
Department of Veterinary Anatomy, Faculty of Agriculture, Tottori University, Japan.
Nihon Juigaku Zasshi. 1990 Jun;52(3):503-12. doi: 10.1292/jvms1939.52.503.
The development of the pecten oculi, a structure peculiar to the avian eye, was studied by scanning electron microscopy (SEM) correlated with light microscopy (LM) in embryonic and adult chickens. The development of the chick pecten was divided into 4 phases: (1) formation of the primordial pecten (Hamburger-Hamilton's stages 27 to 29), (2) formation of the plate-like pecten (stages 30 to 34), (3) pleat formation and pigmentation (stages 35 to 37), and (4) bridge formation and high-vascularization (stage 38 to adult). The primordial pecten is formed entirely from the ectoderm by fusion of the inwardly-projecting edges of the optic fissure. The primordial pecten grows into a tall, thin plate rising from a broad base. The pecten begins to fold slightly at stage 35. The number of pleats increases rapidly, from 7 at stage 35, to 16 at stage 36, 18 at stage 37, and 19 to 20 at stage 40. The bridge begins to form at stage 38 by a swelling of the apical edge of the pecten and completes its development by the twentieth post-hatch day. Blood vessels appear first in the broad base of the plate-like pecten, then become more numerous and gradually extend into the pleats. The pecten becomes more vascular than cellular at stage 43, and it is highly vascularized in the adult. The pleat surface becomes conspicuously irregular with increased vascularization. The peripectinate cells, located on the pecten, are already present at stage 27.
利用扫描电子显微镜（SEM）并结合光学显微镜（LM），对鸡胚胎和成年鸡眼部特有的栉膜结构的发育进行了研究。鸡栉膜的发育分为4个阶段：（1）原始栉膜形成阶段（汉伯格-汉密尔顿第27至29期），（2）板状栉膜形成阶段（第30至34期），（3）褶皱形成和色素沉着阶段（第35至37期），以及（4）桥形成和高度血管化阶段（第38期至成年期）。原始栉膜完全由视裂向内突出边缘融合形成的外胚层构成。原始栉膜从宽阔的基部生长成一个高而薄的板状结构。栉膜在第35期开始轻微折叠。褶皱数量迅速增加，从第35期的7条增加到第36期的16条、第37期的18条以及第40期的19至20条。桥在第38期通过栉膜顶端边缘肿胀开始形成，并在孵化后第20天完成发育。血管首先出现在板状栉膜的宽阔基部，然后数量增多并逐渐延伸至褶皱中。栉膜在第43期时血管化程度超过细胞化程度，在成年时高度血管化。随着血管化增加，褶皱表面变得明显不规则。位于栉膜上的栉周细胞在第27期就已出现。
