Helmholtz Centre of Environmental Research-UFZ, Department of Environmental Microbiology, Leipzig, Germany.
Appl Environ Microbiol. 2013 Sep;79(18):5777-81. doi: 10.1128/AEM.01598-13. Epub 2013 Jul 12.
Soil microeukaryotes may trophically benefit from plant biopolymers. However, carbon transfer from cellulose into soil microeukaryotes has not been demonstrated so far. Microeukaryotes assimilating cellulose-derived carbon in oxic and anoxic soil slurries were therefore examined by rRNA-based stable-isotope probing. Bacteriovorous flagellates and ciliates and, likely, mixotrophic algae and saprotrophic fungi incorporated carbon from supplemental [U-(13)C]cellulose under oxic conditions. A previous study using the same soil suggested that cellulolytic Bacteria assimilated (13)C of supplemental cellulose. Thus, it can be assumed that ciliates, cercozoa, and chrysophytes assimilated carbon by grazing upon and utilizing metabolic products of Bacteria that hydrolyzed cellulose in the soil slurries.
土壤微型真核生物可能从植物生物聚合物中获得营养益处。然而，迄今为止尚未证明纤维素中的碳向土壤微型真核生物的转移。因此，通过基于 rRNA 的稳定同位素探测研究了在有氧和缺氧土壤悬浮液中同化纤维素衍生碳的微型真核生物。在有氧条件下，细菌鞭毛虫和纤毛虫以及可能的混合营养藻类和腐生真菌从补充的[U-(13)C]纤维素中吸收了碳。先前使用相同土壤的一项研究表明，纤维素分解细菌同化了补充纤维素的(13)C。因此，可以假设纤毛虫、原生动物和金藻通过吞噬和利用土壤悬浮液中纤维素水解细菌的代谢产物来同化碳。
