Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109-5622, USA.
Pancreas. 2013 Aug;42(6):905-11. doi: 10.1097/MPA.0b013e31828fddc3.
Proteomics is an approach to looking at the identity, amount, proteolysis, compartmentalization, and posttranslational modification of a large number of proteins simultaneously in a cell or tissue. Recently, proteomics has begun to be applied to the study of pancreatitis to ascertain mechanisms of disease and search for biomarkers of disease. Most mechanistic work has been carried out in animal models of acute pancreatitis. In 8 studies, 97 proteins have been reported to increase, 55 to decrease, and 23 to undergo proteolysis. Proteins showing increases are most often related to stress, inflammation, or the cytoskeleton, whereas decreases are seen in digestive enzymes and proteins related to metabolism. Many protein changes however, are not consistent between studies and only the most recent studies are rigorous and quantitative. By contrast, biomarker studies have focused on pancreatic juice and plasma of humans with disease and often are directed at distinguishing chronic pancreatitis from cancer. Chronic pancreatitis has also been investigated in tissue sections of histological samples. In this review, the results of studies to date are described as well as coverage of the methods used and special issues that must be considered. Areas are pointed out that are worthy of future study.
蛋白质组学是一种同时在细胞或组织中观察大量蛋白质的身份、数量、蛋白水解、区室化和翻译后修饰的方法。最近，蛋白质组学开始应用于胰腺炎的研究，以确定疾病的机制并寻找疾病的生物标志物。大多数机制研究都是在急性胰腺炎的动物模型中进行的。在 8 项研究中，报告了 97 种蛋白质增加，55 种蛋白质减少，23 种蛋白质发生蛋白水解。增加的蛋白质通常与应激、炎症或细胞骨架有关，而减少的则是消化酶和与代谢有关的蛋白质。然而，许多蛋白质变化在研究之间并不一致，只有最近的研究才具有严格的定量性。相比之下，生物标志物研究集中在有疾病的人类的胰液和血浆上，并且通常旨在区分慢性胰腺炎和癌症。慢性胰腺炎也在组织切片的组织样本中进行了研究。在这篇综述中，描述了迄今为止的研究结果，以及所使用的方法和必须考虑的特殊问题的涵盖范围。指出了值得未来研究的领域。
