Department of Gastroenterology, Fudan University School of Medicine, Shanghai, China.
Pancreas. 2012 Mar;41(2):317-22. doi: 10.1097/MPA.0b013e31822981fb.
Hyperlipidemia is associated with a variety of pancreatic diseases. However, the underlying pathophysiology and molecular mechanisms between hyperlipidemia and acute pancreatitis remain undefined. Gel electrophoresis and mass spectrometry can be used in proteomic analysis to elucidate these mechanisms.
A comparative proteomic analysis was conducted to identify proteins that were altered in pancreases of hyperlipidemic acute necrotic pancreatitis rats compared with those of normal-lipid acute necrotic pancreatitis rats. A comparative proteomic approach using a hyperlipidemic rat model was used.
Thirty-nine differentially expressed proteins were significantly changed in pancreatic samples from hyperlipidemic acute necrotic pancreatitis rats. Differentially expressed proteins in hyperlipidemic pancreatitis include pancreatic proteolytic enzymes, such as lipase, amylase, carboxypolypeptidase, and α-1-antiproteinase; endoplasmic reticulum stress-related proteins; and calcium influx-related proteins including protein disulfide isomerase, calreticulin, annexin A, glucose-regulated protein 78, heat shock protein 60, and peroxiredoxin. Other proteins associated with DNA replication and damage repair, apoptosis, cell metabolism, circulatory dysfunction, and signal transduction were identified in hyperlipidemic pancreatitis.
Hyperlipidemia intensifies acute necrotic pancreatitis through various ways. These enzymes may be putative biomarkers of hyperlipidemic acute necrotic pancreatitis.
高血脂与多种胰腺疾病有关。然而，高血脂与急性胰腺炎之间的潜在病理生理学和分子机制尚不清楚。凝胶电泳和质谱分析可用于蛋白质组学分析，以阐明这些机制。
采用比较蛋白质组学分析方法，比较高脂血症急性坏死性胰腺炎大鼠胰腺与正常脂质急性坏死性胰腺炎大鼠胰腺中差异表达的蛋白质。采用高脂血症大鼠模型进行比较蛋白质组学研究。
在高脂血症急性坏死性胰腺炎大鼠的胰腺样本中，有 39 种差异表达蛋白发生了显著变化。高脂血症性胰腺炎中的差异表达蛋白包括胰蛋白酶、淀粉酶、羧肽酶和α-1-抗胰蛋白酶等胰腺蛋白水解酶；内质网应激相关蛋白；以及钙内流相关蛋白，包括蛋白二硫键异构酶、钙网蛋白、膜联蛋白 A、葡萄糖调节蛋白 78、热休克蛋白 60 和过氧化物酶。还鉴定出与 DNA 复制和损伤修复、细胞凋亡、细胞代谢、循环功能障碍和信号转导相关的其他蛋白质。
高脂血症通过多种途径加重急性坏死性胰腺炎。这些酶可能是高脂血症性急性坏死性胰腺炎的潜在生物标志物。
