淀粉样β肽和神经节苷脂对小鼠神经干细胞的影响。
Effects of amyloid β-peptides and gangliosides on mouse neural stem cells.
机构信息
Institute of Molecular Medicine and Genetics and Institute of Neuroscience, Medical College of Georgia, Georgia Regents University, Augusta, GA, 30912, USA.
出版信息
Neurochem Res. 2013 Oct;38(10):2019-27. doi: 10.1007/s11064-013-1108-y. Epub 2013 Jul 14.
The interaction of amyloid β-proteins (Aβs) with membrane lipids has been postulated as an early event in Aβ fibril formation in Alzheimer's disease. We evaluated the effects of several putative bioactive Aβs and gangliosides on neural stem cells (NSCs) isolated from embryonic mouse brains or the subventricular zone of adult mouse brains. Incubation of the isolated NSCs with soluble Aβ1-40 alone did not cause any change in the number of NSCs, but soluble Aβ1-42 increased their number. Aggregated Aβ1-40 and Aβ1-42 increased the number of NSCs but soluble and aggregated Aβ25-35 decreased the number. Soluble Aβ1-40 and Aβ1-42 did not affect the number of apoptotic cells but aggregated Aβ1-40 and Aβ1-42 did. When NSCs were treated with a combination of GM1 or GD3 and soluble Aβ1-42, cell proliferation was enhanced, indicating that both GM1 and GD3 as well as Aβs are involved in promoting cell proliferation and survival of NSCs. These observations suggest the potential of beneficial effects of using gangliosides and Aβs for promoting NSC proliferation.
淀粉样β-蛋白（Aβs）与膜脂的相互作用被认为是阿尔茨海默病中 Aβ 纤维形成的早期事件。我们评估了几种假定的生物活性 Aβ 和神经节苷脂对从胚胎鼠脑中分离的神经干细胞（NSCs）或成年鼠脑室下区的 NSCs 的影响。单独孵育分离的 NSCs 与可溶性 Aβ1-40 不会引起 NSCs 数量的任何变化，但可溶性 Aβ1-42 增加了它们的数量。聚集的 Aβ1-40 和 Aβ1-42 增加了 NSCs 的数量，但可溶性和聚集的 Aβ25-35 减少了 NSCs 的数量。可溶性 Aβ1-40 和 Aβ1-42 不影响细胞凋亡的数量，但聚集的 Aβ1-40 和 Aβ1-42 会影响。当 NSCs 用 GM1 或 GD3 与可溶性 Aβ1-42 联合处理时，细胞增殖增强，表明 GM1 和 GD3 以及 Aβ 都参与促进 NSCs 的增殖和存活。这些观察结果表明，使用神经节苷脂和 Aβ 促进 NSCs 增殖可能具有有益效果。
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