Lan Jin, Guo Pin, Chen Mo, Wu Bin, Mao Qing, Qiu Yongming
Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Craniofac Surg. 2013 Jul;24(4):1441-6. doi: 10.1097/SCS.0b013e318295378b.
O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 (PomGnT1) constitutes one third of the O-linked glycoproteins in brain tissue. However, its functions have been seldom investigated in brain cancers. In this study, immunohistochemistry was used for the detection of the PomGnT1 protein in 133 cases of glioma tissues. Spearman correlation analysis was used for the relationship between PomGnT1 staining and the glioma grade. Receiver operating characteristic curve was used to measure the diagnostic value of PomGnT1 protein in the degree of glioma malignance. We found that PomGnT1 expression was correlated with glioma grade, and it could be used as a marker to distinguish low- and high-grade gliomas. Stably transfected U87 cells were constructed to overexpress short hairpin RNA of PomGnT1. Immunofluorescence test detected that this protein also could restrain the generation of U87 cells' pseudopodia. Western blotting further showed that the PomGnT1 protein had an impact on the c-myc protein level. In conclusion, our data suggest that PomGnT1 protein was correlated with the malignance of glioma progression, the mechanism involved in glioma cell's pseudopodium formation, and the expression of c-myc protein.
O-连接甘露糖β-1,2-N-乙酰葡糖胺基转移酶1（PomGnT1）占脑组织中O-连接糖蛋白的三分之一。然而，其在脑癌中的功能很少被研究。在本研究中，采用免疫组织化学法检测133例胶质瘤组织中的PomGnT1蛋白。采用Spearman相关性分析研究PomGnT1染色与胶质瘤分级之间的关系。采用受试者工作特征曲线来衡量PomGnT1蛋白在胶质瘤恶性程度中的诊断价值。我们发现PomGnT1表达与胶质瘤分级相关，并且它可以作为区分低级别和高级别胶质瘤的标志物。构建稳定转染的U87细胞以过表达PomGnT1的短发夹RNA。免疫荧光检测发现该蛋白还可以抑制U87细胞伪足的产生。蛋白质印迹进一步表明PomGnT1蛋白对c-myc蛋白水平有影响。总之，我们的数据表明PomGnT1蛋白与胶质瘤进展的恶性程度、胶质瘤细胞伪足形成的机制以及c-myc蛋白的表达相关。
