[Evaluation of the biocompatibility and cell segregation performance of acellular dermal matrix as barrier membrane on guided tissue regeneration in vitro].

PURPOSE

To investigate the proliferation of human periodontal ligament cell on acellular dermal matrix (ADM) and the epithelial cell segregation performance of ADM and evaluate the feasibility of ADM as barrier membrane of guided tissue regeneration.

METHODS

Human periodontal ligament cells(HPDLCs) of the 3rd to 5th passage were seeded onto 96-well plates(with ADM and e-PTFE inside) with 2000 cells per well. The cells were cultured in Dulbecco's modified eagle medium (DMEM). The MTT colorimetric assay method was performed at day 1, 3, 5 and 7 after incubation. The optical density(OD) of each well was measured spectrophotometrically at 490 nm to monitor effects on cell proliferation. The data was analyzed using Student's t test by SPSS13.0 software package. In addition, Tca8113 cells were placed in 24-well plates (with ADM and e-PTFE inside) with 2×10(4) cells per well. The DAPI staining was done 5, 10 d after incubation. Fluorescence microscope was used to observe the number of cells which lied on the two sides of the materials. Visual field was randomly selected to record the number of cells. The cell inoculated surface was recorded as ADM group and e-PTFE group, the other surface was recorded as ADM group and e-PTFE group. Student's t test was used to analyse the cell segregation of the two membranes.

RESULTS

At 3-, 5-, 7 d, the OD value of ADM group and blank control group was significantly higher than that in e-PTFE group (P<0.05), no significant difference was found between ADM group and blank control group (P>0.05). At 5-, 10 d, the cell number in ADM group was much more than that in ADM group, similar between e-PTFE group and e-PTFE group (P<0.05), while no significant difference was noted between the ADM group and e-PTFE group (P>0.05).

CONCLUSIONS

ADM is more conducive to the proliferation of HPDLCs than e-PTFE, and has the similar cell segregation performance on the epithelial cells. Compared with e-PTFE, ADM is more suitable for guided periodontal tissue regeneration. Supported by Health Science and Technology Projects of Jiangsu Province(H201231) and Priority Academic Program Development of Jiangsu Higher Education Institutions (2011-137).