接种于脱细胞真皮基质（3-D ADM）上的原代鸡胚成纤维细胞可促进大鼠全层皮肤伤口的再生。

Primary chicken embryo fibroblasts seeded acellular dermal matrix (3-D ADM) improve regeneration of full thickness skin wounds in rats.

作者信息

Gangwar Anil Kumar, Kumar Naveen, Khangembam Sangeeta Devi, Kumar Vineet, Singh Rajendra

机构信息

Division of Surgery, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India; Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad 224229, Uttar Pradesh, India.

Division of Surgery, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India.

出版信息

Tissue Cell. 2015 Jun;47(3):311-22. doi: 10.1016/j.tice.2015.04.002. Epub 2015 Apr 11.

DOI:10.1016/j.tice.2015.04.002

PMID:25907656

Abstract

Rat skins were deepithelialized and decellularized by hypertonic saline and sodium deoxycholate (SDC), respectively. Primary chicken embryo fibroblasts (P-CEF) were cultured and seeded on prepared acellular dermal matrix (ADM). A full thickness skin defect (20×20 mm(2)) was created in thirty-six rats and randomly divided into three equal groups. Defect was left open, repaired with ADM and ADM seeded with P-CEF (3-D ADM) in groups 1, 2 and 3, respectively. By day 28, the treated wounds healed completely without scar. By day 7 hydroxyproline contents was higher in group 3 as compared to groups 1 and 2. There was slightly more B cell response in animals implanted with ADM and 3-D ADM. At day 21, stimulation index was lower with acellular dermis antigen as compared to 3-D ADM antigen. In group 1 on day 3, the granulation tissue showed more inflammatory reaction, fibroplasia and neovascularization as compared to group 2 and 3. By day 28, there was complete epithelization was observed in all groups over. However, a large scar was observed in group 1. The graft was completely absorbed and replaced with densely thick and best arranged collagen fibers. On day 7, malonyldialdehyde and superoxide dismutase levels were significantly (P<0.05) increased in group 1. Reduced glutathione values increased and reached to near normal in groups 2 and 3. Catalase values were significantly (P<0.05) higher in group 1 at different time intervals. SEM samples of group 2 showed ingrowth of fibroblasts into acellular matrix at host graft junction. However, in group 3 fibroblasts were infiltrated within the pores of graft. It was concluded that P-CEF cells seeded ADM facilitated early and better healing.

摘要

分别用高渗盐水和脱氧胆酸钠（SDC）对大鼠皮肤进行去上皮和脱细胞处理。培养原代鸡胚成纤维细胞（P-CEF），并接种到制备好的无细胞真皮基质（ADM）上。在36只大鼠身上制造全层皮肤缺损（20×20 mm²），并随机分为三组。第1、2和3组的缺损分别不做处理、用ADM修复以及用接种了P-CEF的ADM（3-D ADM）修复。到第28天，治疗的伤口完全愈合且无瘢痕。到第7天，第3组的羟脯氨酸含量高于第1组和第2组。植入ADM和3-D ADM的动物中B细胞反应略多。在第21天，与3-D ADM抗原相比，无细胞真皮抗原的刺激指数较低。在第3天，第1组的肉芽组织与第2组和第3组相比，炎症反应、纤维组织增生和新血管形成更多。到第28天，所有组均观察到完全上皮化。然而，第1组观察到一个大瘢痕。移植物被完全吸收，取而代之的是密集增厚且排列最佳的胶原纤维。在第7天，第1组的丙二醛和超氧化物歧化酶水平显著（P<0.05）升高。第2组和第3组的还原型谷胱甘肽值升高并接近正常。在不同时间间隔，第1组的过氧化氢酶值显著（P<0.05）更高。第2组的扫描电子显微镜样本显示成纤维细胞在宿主移植物交界处向内生长到无细胞基质中。然而，在第3组中，成纤维细胞浸润在移植物的孔隙内。得出的结论是，接种了P-CEF细胞的ADM促进了早期且更好的愈合。