顺二氯二氨铂（II）诱导培养的人淋巴细胞中的姐妹染色单体交换和染色体畸变形成及其被硫代硫酸钠抑制的情况。

cis-diamminedichloroplatinum(II)-induced sister-chromatid exchanges and chromosome aberration formation in cultured human lymphocytes and their inhibition by sodium thiosulfate.

作者信息

Ohe T, Tsuda S, Sakata Y, Taniwaki M, Misawa S, Abe T

机构信息

Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.

出版信息

Mutat Res. 1990 Aug;244(4):279-85. doi: 10.1016/0165-7992(90)90073-s.

DOI:10.1016/0165-7992(90)90073-s

PMID:2385243

Abstract

cis-Diamminedichloroplatinum(II) (cis-DDP)-induced sister-chromatid exchanges (SCEs) and chromosome aberration formation were studied in human lymphocytes. The mitotic index decreased abruptly at 2 X 10(-6) M cis-DDP and the frequency of SCEs was dose-related; a marked increase was recorded at 10(-6) M cis-DDP. A dose-dependent effect was also found for chromosome aberration formation at concentrations between 10(-11) and 4 X 10(-6) M. The aberrations observed were primarily chromatid breaks and gaps. We also examined the inhibition of these genotoxicities by treating the cells with sodium thiosulfate (STS). Simultaneous treatment with 10(-4)-10(-3) M STS (100-1000-fold molar ratio to cis-DDP) significantly reduced the frequency of SCEs induced by 10(-6) M cis-DDP. Furthermore, a 3-h delay in treating with STS significantly reduced cis-DDP-induced SCEs, but not chromosome aberration formation.

摘要

研究了顺二氯二氨铂（II）（顺铂）诱导的人淋巴细胞姐妹染色单体交换（SCEs）和染色体畸变形成。在2×10^(-6) M顺铂浓度下，有丝分裂指数急剧下降，且SCEs频率与剂量相关；在10^(-6) M顺铂浓度下记录到显著增加。在10^(-11)至4×10^(-6) M浓度范围内，染色体畸变形成也呈现剂量依赖性效应。观察到的畸变主要是染色单体断裂和裂隙。我们还通过用硫代硫酸钠（STS）处理细胞来检测其对这些遗传毒性的抑制作用。与10^(-4) - 10^(-3) M STS同时处理（与顺铂的摩尔比为100 - 1000倍）可显著降低10^(-6) M顺铂诱导的SCEs频率。此外，延迟3小时用STS处理可显著降低顺铂诱导的SCEs，但对染色体畸变形成无影响。