Molecular Plant Nutrition, Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany.
Plant Physiol. 2013 Sep;163(1):161-79. doi: 10.1104/pp.113.218453. Epub 2013 Jul 12.
Plant roots show a particularly high variation in their morphological response to different nutrient deficiencies. Although such changes often determine the nutrient efficiency or stress tolerance of plants, it is surprising that a comprehensive and comparative analysis of root morphological responses to different nutrient deficiencies has not yet been conducted. Since one reason for this is an inherent difficulty in obtaining nutrient-deficient conditions in agar culture, we first identified conditions appropriate for producing nutrient-deficient plants on agar plates. Based on a careful selection of agar specifically for each nutrient being considered, we grew Arabidopsis (Arabidopsis thaliana) plants at four levels of deficiency for 12 nutrients and quantified seven root traits. In combination with measurements of biomass and elemental concentrations, we observed that the nutritional status and type of nutrient determined the extent and type of changes in root system architecture (RSA). The independent regulation of individual root traits further pointed to a differential sensitivity of root tissues to nutrient limitations. To capture the variation in RSA under different nutrient supplies, we used principal component analysis and developed a root plasticity chart representing the overall modulations in RSA under a given treatment. This systematic comparison of RSA responses to nutrient deficiencies provides a comprehensive view of the overall changes in root plasticity induced by the deficiency of single nutrients and provides a solid basis for the identification of nutrient-sensitive steps in the root developmental program.
植物根系在对不同养分缺乏的形态响应上表现出特别高的变异性。尽管这些变化通常决定了植物的养分效率或抗胁迫能力，但令人惊讶的是，对不同养分缺乏的根系形态响应的综合和比较分析尚未进行。由于在琼脂培养中获得养分缺乏条件存在固有困难是其中一个原因，我们首先确定了在琼脂平板上生产养分缺乏植物的适宜条件。基于对每种考虑的养分专门选择琼脂，我们在 12 种养分的四个缺乏水平下种植拟南芥（Arabidopsis thaliana）植物，并量化了 7 种根系特征。结合生物量和元素浓度的测量，我们观察到营养状况和养分类型决定了根系系统结构（RSA）变化的程度和类型。个别根系特征的独立调节进一步表明根组织对养分限制的敏感性存在差异。为了捕捉不同养分供应下 RSA 的变化，我们使用主成分分析并开发了一个根系可塑性图表，代表给定处理下 RSA 的整体调节。对 RSA 对养分缺乏的响应的系统比较提供了对单个养分缺乏引起的根系可塑性整体变化的全面了解，并为确定根系发育程序中对养分敏感的步骤提供了坚实的基础。
