• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

侧根发育的定量分析:陷阱及规避方法。

Quantitative analysis of lateral root development: pitfalls and how to avoid them.

机构信息

Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62250 Cuernavaca, Morelos, Mexico.

出版信息

Plant Cell. 2012 Jan;24(1):4-14. doi: 10.1105/tpc.111.089698. Epub 2012 Jan 6.

DOI:10.1105/tpc.111.089698
PMID:22227889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3289558/
Abstract

The advent of the postgenomics era has led to increased interest in exploring the role of gene networks and signaling pathways in controlling plant development. The last two decades have seen a particular increase in the number of studies focusing on the development of the Arabidopsis thaliana root system. However, the investigation of such a seemingly simple system as an Arabidopsis root can lead to problems in quantification and errors in interpretation if knowledge of root organization is lacking. In this article, we identify a number of these problems and give examples of potentially erroneous and correct determinations of lateral root parameters. Our aim is to bring this important issue to the attention of the plant science community and to suggest ways in which the problems inherent in quantifying the process of lateral root development can be avoided.

摘要

后基因组时代的到来使得人们越来越关注基因网络和信号通路在控制植物发育中的作用。过去二十年中,越来越多的研究聚焦于拟南芥根系的发育。然而,如果缺乏对根系组织的了解,那么研究这样一个看似简单的系统(如拟南芥的根系)可能会导致定量方面的问题和解释上的错误。在本文中,我们确定了其中的一些问题,并给出了一些潜在错误和正确的侧根参数确定的例子。我们的目的是引起植物科学界对这个重要问题的关注,并提出避免量化侧根发育过程中固有问题的方法。

相似文献

1
Quantitative analysis of lateral root development: pitfalls and how to avoid them.侧根发育的定量分析:陷阱及规避方法。
Plant Cell. 2012 Jan;24(1):4-14. doi: 10.1105/tpc.111.089698. Epub 2012 Jan 6.
2
Hormonal regulation of lateral root development in Arabidopsis modulated by MIZ1 and requirement of GNOM activity for MIZ1 function.拟南芥侧根发育的激素调控受 MIZ1 调节,并且 GNOM 活性是 MIZ1 功能所必需的。
Plant Physiol. 2011 Nov;157(3):1209-20. doi: 10.1104/pp.111.186270. Epub 2011 Sep 22.
3
Small-molecule screens to study lateral root development.用于研究侧根发育的小分子筛选
Methods Mol Biol. 2013;959:189-95. doi: 10.1007/978-1-62703-221-6_12.
4
Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent signaling mechanism in Arabidopsis thaliana.巨大芽孢杆菌根际细菌通过一种不依赖生长素和乙烯的信号传导机制促进拟南芥生长并改变其根系结构。
Mol Plant Microbe Interact. 2007 Feb;20(2):207-17. doi: 10.1094/MPMI-20-2-0207.
5
Talking through walls: mechanisms of lateral root emergence in Arabidopsis thaliana.穿透墙壁的对话:拟南芥侧根发生的机制。
Curr Opin Plant Biol. 2015 Feb;23:31-8. doi: 10.1016/j.pbi.2014.10.005. Epub 2014 Oct 30.
6
Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana.拟南芥侧根发育的遗传与表型分析
Methods Mol Biol. 2018;1761:47-75. doi: 10.1007/978-1-4939-7747-5_4.
7
Quantitative trait loci controlling root growth and architecture in Arabidopsis thaliana confirmed by heterogeneous inbred family.通过异质近交家系证实的拟南芥中控制根系生长和结构的数量性状基因座。
Theor Appl Genet. 2005 Feb;110(4):742-53. doi: 10.1007/s00122-004-1900-9. Epub 2005 Jan 28.
8
Control of Arabidopsis root development.拟南芥根系发育的调控。
Annu Rev Plant Biol. 2012;63:563-90. doi: 10.1146/annurev-arplant-042811-105501. Epub 2012 Feb 9.
9
The role of root system architecture and root hairs in promoting anchorage against uprooting forces in Allium cepa and root mutants of Arabidopsis thaliana.根系结构和根毛在促进洋葱和拟南芥根突变体抵抗拔根力方面的作用。
J Exp Bot. 2002 Feb;53(367):333-40. doi: 10.1093/jexbot/53.367.333.
10
Glucose and auxin signaling interaction in controlling Arabidopsis thaliana seedlings root growth and development.葡萄糖与生长素信号转导在调控拟南芥幼苗根系生长发育中的相互作用
PLoS One. 2009;4(2):e4502. doi: 10.1371/journal.pone.0004502. Epub 2009 Feb 18.

引用本文的文献

1
ARABIDOPSIS HOMOLOG OF TRITHORAX1 impacts lateral root development by epigenetic regulation of targets involved in root system architecture.TRITHORAX1的拟南芥同源物通过对参与根系结构的靶标进行表观遗传调控来影响侧根发育。
New Phytol. 2025 Sep;247(5):2180-2195. doi: 10.1111/nph.70349. Epub 2025 Jul 7.
2
BZR1 promotes pluripotency acquisition and callus development through direct regulation of ARF7 and ARF19.BZR1通过直接调控ARF7和ARF19促进多能性获得和愈伤组织发育。
EMBO Rep. 2025 Jun 26. doi: 10.1038/s44319-025-00433-5.
3
The TaCLE24b peptide signaling cascade modulates lateral root development and drought tolerance in wheat.TaCLE24b肽信号级联调节小麦侧根发育和耐旱性。
Nat Commun. 2025 Feb 25;16(1):1952. doi: 10.1038/s41467-025-57291-x.
4
The calcium sensor AtCML8 contributes to Arabidopsis plant cell growth by modulating the brassinosteroid signaling pathway.钙传感器AtCML8通过调节油菜素内酯信号通路促进拟南芥植物细胞生长。
Plant J. 2025 Jan;121(1):e17179. doi: 10.1111/tpj.17179. Epub 2024 Dec 2.
5
Exogenous application of the apocarotenoid retinaldehyde negatively regulates auxin-mediated root growth.外源性视黄醛作为类胡萝卜素可负调控生长素介导的根生长。
Plant Physiol. 2024 Oct 1;196(2):1659-1673. doi: 10.1093/plphys/kiae405.
6
NBR1-mediated selective autophagy of ARF7 modulates root branching.NBR1 介导的 ARF7 选择性自噬调节根分枝。
EMBO Rep. 2024 Jun;25(6):2571-2591. doi: 10.1038/s44319-024-00142-5. Epub 2024 Apr 29.
7
A biostimulant yeast, Hanseniaspora opuntiae, modifies Arabidopsis thaliana root architecture and improves the plant defense response against Botrytis cinerea.一种生物刺激酵母,仙人掌汉逊酵母,可改变拟南芥的根系结构,并增强植物对灰葡萄孢的防御反应。
Planta. 2024 Jan 31;259(3):53. doi: 10.1007/s00425-023-04326-6.
8
An improved and convenient petri plate-based method for studying the root growth of plants.一种经过改进的、便捷的基于培养皿研究植物根系生长的方法。
MethodsX. 2023 Nov 30;12:102505. doi: 10.1016/j.mex.2023.102505. eCollection 2024 Jun.
9
Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana.海藻糖-6-磷酸信号调节拟南芥侧根的形成。
Proc Natl Acad Sci U S A. 2023 Oct 3;120(40):e2302996120. doi: 10.1073/pnas.2302996120. Epub 2023 Sep 25.
10
Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots to Pseudomonas oryzihabitans.非易失性信号和氧化还原机制是拟南芥根系对水稻黄单胞菌响应所必需的。
J Exp Bot. 2022 Nov 2;73(19):6971-6982. doi: 10.1093/jxb/erac346.

本文引用的文献

1
Auxin minimum defines a developmental window for lateral root initiation.生长素最小浓度定义了侧根起始的发育窗口。
New Phytol. 2011 Sep;191(4):970-983. doi: 10.1111/j.1469-8137.2011.03757.x. Epub 2011 May 13.
2
Transkingdom signaling based on bacterial cyclodipeptides with auxin activity in plants.基于细菌环二肽和植物中生长素活性的跨王国信号转导。
Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7253-8. doi: 10.1073/pnas.1006740108. Epub 2011 Apr 11.
3
The Arabidopsis D-type cyclin CYCD2;1 and the inhibitor ICK2/KRP2 modulate auxin-induced lateral root formation.拟南芥 D 型细胞周期蛋白 CYCD2;1 和抑制剂 ICK2/KRP2 调节生长素诱导的侧根形成。
Plant Cell. 2011 Feb;23(2):641-60. doi: 10.1105/tpc.110.080002. Epub 2011 Feb 25.
4
Serotonin, a tryptophan-derived signal conserved in plants and animals, regulates root system architecture probably acting as a natural auxin inhibitor in Arabidopsis thaliana.血清素是一种在植物和动物中保守的色氨酸衍生信号,它可能作为一种天然的生长素抑制剂在拟南芥中调节根系的结构。
Plant Cell Physiol. 2011 Mar;52(3):490-508. doi: 10.1093/pcp/pcr006. Epub 2011 Jan 19.
5
The heterozygous abp1/ABP1 insertional mutant has defects in functions requiring polar auxin transport and in regulation of early auxin-regulated genes.杂合 abp1/ABP1 插入突变体在需要极性生长素运输的功能和早期生长素调节基因的调控中存在缺陷。
Plant J. 2011 Jan;65(2):282-94. doi: 10.1111/j.1365-313X.2010.04420.x.
6
Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco.细胞分裂素的根特异性减少导致拟南芥和烟草的根系生长增强、耐旱性提高和叶片矿物质富集。
Plant Cell. 2010 Dec;22(12):3905-20. doi: 10.1105/tpc.109.072694. Epub 2010 Dec 10.
7
Auxin-induced inhibition of lateral root initiation contributes to root system shaping in Arabidopsis thaliana.生长素诱导的侧根起始抑制有助于拟南芥根系形态建成。
Plant J. 2010 Dec;64(5):740-52. doi: 10.1111/j.1365-313X.2010.04365.x. Epub 2010 Oct 15.
8
ABI4 mediates abscisic acid and cytokinin inhibition of lateral root formation by reducing polar auxin transport in Arabidopsis.ABI4 通过减少拟南芥中极性生长素的运输,介导脱落酸和细胞分裂素对侧根形成的抑制。
Plant Cell. 2010 Nov;22(11):3560-73. doi: 10.1105/tpc.110.074641. Epub 2010 Nov 19.
9
Strigolactones affect lateral root formation and root-hair elongation in Arabidopsis.独脚金内酯影响拟南芥侧根形成和根毛伸长。
Planta. 2011 Jan;233(1):209-16. doi: 10.1007/s00425-010-1310-y. Epub 2010 Nov 16.
10
Arabidopsis Tyrosylprotein sulfotransferase acts in the auxin/PLETHORA pathway in regulating postembryonic maintenance of the root stem cell niche.拟南芥酪氨酰蛋白硫酸转移酶在生长素/PLT 途径中作用,调节根干细胞龛的胚胎后维持。
Plant Cell. 2010 Nov;22(11):3692-709. doi: 10.1105/tpc.110.075721. Epub 2010 Nov 2.