Breyton Cécile, Gabel Frank, Lethier Mathilde, Flayhan Ali, Durand Grégory, Jault Jean-Michel, Juillan-Binard Céline, Imbert Lionel, Moulin Martine, Ravaud Stéphanie, Härtlein Michael, Ebel Christine
Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027, Grenoble, France.
Eur Phys J E Soft Matter. 2013 Jul;36(7):71. doi: 10.1140/epje/i2013-13071-6. Epub 2013 Jul 16.
Small angle neutron scattering (SANS) is a powerful technique for investigating association states and conformational changes of biological macromolecules in solution. SANS is of particular interest for the study of the multi-component systems, as membrane protein complexes, for which in vitro characterisation and structure determination are often difficult. This article details the important physical properties of surfactants in view of small angle neutron scattering studies and the interest to deuterate membrane proteins for contrast variation studies. We present strategies for the production of deuterated membrane proteins and methods for quality control. We then review some studies on membrane proteins, and focus on the strategies to overcome the intrinsic difficulty to eliminate homogeneously the detergent or surfactant signal for solubilised membrane proteins, or that of lipids for membrane proteins inserted in liposomes.
小角中子散射（SANS）是一种用于研究溶液中生物大分子缔合状态和构象变化的强大技术。对于多组分系统的研究，如膜蛋白复合物，SANS特别有意义，因为这类系统的体外表征和结构测定往往很困难。本文鉴于小角中子散射研究详细介绍了表面活性剂的重要物理性质，以及氘代膜蛋白用于对比变化研究的意义。我们提出了生产氘代膜蛋白的策略和质量控制方法。然后我们回顾了一些关于膜蛋白的研究，并重点关注克服内在困难的策略，即如何均匀消除溶解的膜蛋白中去污剂或表面活性剂的信号，或插入脂质体中的膜蛋白中脂质的信号。
