Plantinga Theo S, Joosten Leo A B, Netea Mihai G
Department of Medicine, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.
Methods Mol Biol. 2013;1040:29-39. doi: 10.1007/978-1-62703-523-1_4.
Inflammasomes are central regulators of inflammation, responsible for cleavage of the inactive pro--inflammatory cytokines IL-1β and IL-18 into their biologically active counterparts. Several regulatory stages within the pathways responsible for the production of these cytokines have been identified. In this chapter, methods are described for assessing the degree of activity of these regulatory stages, which include mRNA transcription, caspase-1 activation, and secretion of the bioactive proteins.
炎性小体是炎症的核心调节因子，负责将无活性的促炎细胞因子白细胞介素-1β（IL-1β）和白细胞介素-18（IL-18）切割成其生物活性形式。已经确定了这些细胞因子产生途径中的几个调节阶段。在本章中，将描述评估这些调节阶段活性程度的方法，包括mRNA转录、半胱天冬酶-1激活以及生物活性蛋白的分泌。
