Cell Biology Unit, IRCCS AOU San Martino IST, 16132 Genova, Italy.
Semin Immunol. 2013 Dec 15;25(6):425-9. doi: 10.1016/j.smim.2013.10.007. Epub 2013 Nov 5.
Unlike most cytokines, IL-1β lacks a secretory signal sequence raising the question of how is this cytokine processed and delivered outside the producing cells. After the seminal observation that IL-1β is actively secreted by human monocytes through a route alternative to the classic endoplasmic reticulum-Golgi, several different pathways have been proposed for IL-1β secretion in different cell types and culture conditions, some of which are unique to macrophage cell lines. Here we describe the most credited of these pathways. In particular, we will focus on IL-1β secretion from primary human blood monocytes. In fact, although data from macrophages or macrophage cell lines are predominant, secretion of IL-1β by monocytes is the most clinically relevant.
与大多数细胞因子不同，IL-1β缺乏分泌信号序列，这就提出了一个问题，即这种细胞因子是如何在产生细胞外被加工和传递的。在开创性地观察到人类单核细胞通过不同于经典内质网-高尔基体的途径主动分泌 IL-1β之后，已经在不同的细胞类型和培养条件下提出了几种不同的 IL-1β分泌途径，其中一些途径是巨噬细胞系所特有的。在这里，我们将描述其中最可信的途径。特别是，我们将重点介绍从原代人血单核细胞中分泌的 IL-1β。事实上，尽管巨噬细胞或巨噬细胞系的数据占主导地位，但单核细胞分泌的 IL-1β与临床相关性最强。
