Department of Plant Protection, Univesity of Nanjing Agriculture, Nanjing, 210095, China; Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
J Sci Food Agric. 2014 Mar 15;94(4):677-82. doi: 10.1002/jsfa.6304. Epub 2013 Aug 9.
Tomato yellow leaf curl virus disease (TYLCVD) causes severe to economic losses in tomato crops in China. The control of TYLCVD is based primarily on the use of synthetic insecticide to control its vector whitefly (Bemisia tabaci). To look for an alternative method for disease control, we investigated the effect of eugenol on controlling TYLCVD. The potential of eugenol to trigger systemic acquired resistance (SAR) in tomato (Jiangsu 14) plants against TYLCV was also investigated.
In greenhouse experiments, eugenol significantly reduced disease severity when applied as a foliar spray, thus demonstrating a systemic effect. The disease spread rapidly in control plants and by the end of the experiment almost all control plants showed severe symptoms. Eugenol also induced H₂O₂ accumulation in tomato plants. Activities of peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were significantly induced compared with those of control plants. As further consequences, increase of salicylic acid (SA) levels and expression of PR-1 proteins, a molecular marker of SAR in tomato, could also be observed.
This is the first report of eugenol as an elicitor and its ability to suppress plant virus diseases under greenhouse conditions. It is suggested that eugenol has the potential to be an effective biocontrol agent against TYLCV in tomato plants.
番茄黄曲叶病毒病（TYLCVD）在中国番茄作物中造成严重的经济损失。TYLCVD 的防治主要依赖于使用合成杀虫剂来控制其载体烟粉虱（Bemisia tabaci）。为了寻找替代的疾病控制方法，我们研究了丁香酚对控制 TYLCVD 的作用。还研究了丁香酚在番茄（江苏 14 号）植株中引发系统获得性抗性（SAR）的潜力，以对抗 TYLCV。
在温室实验中，丁香酚作为叶面喷雾显著降低了病害严重度，从而表现出系统效应。对照植株中的病害迅速蔓延，到实验结束时，几乎所有对照植株都出现了严重的症状。丁香酚还诱导番茄植株中 H₂O₂的积累。与对照植株相比，过氧化物酶（POD）、多酚氧化酶（PPO）和苯丙氨酸解氨酶（PAL）的活性显著增加。作为进一步的后果，还可以观察到水杨酸（SA）水平的增加和 PR-1 蛋白的表达，这是番茄 SAR 的分子标志物。
这是首次报道丁香酚作为一种诱导剂及其在温室条件下抑制植物病毒病的能力。丁香酚有可能成为番茄中防治 TYLCV 的有效生物防治剂。
