Department of Plant Production and Soil Science, University of Pretoria, Pretoria 0001, South Africa.
Appl Biochem Biotechnol. 2013 Sep;171(2):437-49. doi: 10.1007/s12010-013-0370-4. Epub 2013 Jul 14.
This study was done to identify random amplified polymorphic DNA (RAPD) markers that may associate with seven important traits in tea. Sixty RAPD primers were first screened using 18 cultivars under each of the 7 traits, followed by confirmatory screening of 20 promising primers with 32 tea cultivars. Six RAPD primers generated a total of nine specific bands that associated with six desired traits: black tea quality and tolerance to drought, high temperature, low temperature, Phomopsis theae, and high yield. These markers would allow early identification of plant material with the desired traits that can be advanced to the next stage of selection and enhance targeted choice of breeding stocks with the desirable traits. The nine RAPD markers identified in this study could improve precision and efficiency in tea breeding and selection and are an important contribution towards the establishment of marker-assisted selection in tea breeding programmes.
本研究旨在鉴定与茶的 7 个重要性状相关的随机扩增多态性 DNA（RAPD）标记。首先，使用 7 种性状下的 18 个品种筛选了 60 个 RAPD 引物，然后用 32 个茶树品种对 20 个有希望的引物进行了确认筛选。6 个 RAPD 引物共产生了 9 条与 6 个目标性状相关的特异带：红茶品质和抗旱、耐热、耐寒、茶拟盘多毛孢和高产。这些标记可以早期鉴定具有所需性状的植物材料，从而推进到下一个选择阶段，并增强对具有理想性状的繁殖材料的有针对性选择。本研究中鉴定的 9 个 RAPD 标记可以提高茶树育种和选择的精度和效率，是在茶树育种计划中建立标记辅助选择的重要贡献。
