Li Juan, Jiang Chang-Jun, Wang Zhao-Xia
Biotechnology Center, Anhui Agricultural University, Hefei 230036, China.
Yi Chuan. 2005 Sep;27(5):765-71.
The study was to evaluate the genetic diversity of 69 tea cultivars of the preconcentrated core germplasms of Camellia Sinensis in China by the random amplified polymorphic DNA (RAPD). Among 50 arbitrary primers, 32 primers could generate enough amplified bands for all the strains in this study. Among a total of 348 bands observed, 328 (94.3%)bands were polymorphic in the 69 cultivars tested except additional 20 cultivars. Genetic distances between the cultivars varied from 0.223 to 0.723. The study indicated that the pre-concentrated core germplasms of Camellia Sinensis in China could well represent the whole collection in respect of genetic structure and genetic diversity and genetic distance. At the same time, it was the best option to establish core collection of Camellia Sinensis in China by combining morphological markers with DNA molecular markers.
本研究旨在通过随机扩增多态性DNA(RAPD)技术评估中国茶树预浓缩核心种质69个茶树品种的遗传多样性。在50条随机引物中,有32条引物能为本研究中的所有菌株产生足够的扩增条带。在所观察到的总共348条带中,除另外20个品种外,69个测试品种中有328条带(94.3%)具有多态性。品种间的遗传距离在0.223至0.723之间。研究表明,中国茶树预浓缩核心种质在遗传结构、遗传多样性和遗传距离方面能够很好地代表整个收集群体。同时,将形态学标记与DNA分子标记相结合是建立中国茶树核心种质库的最佳选择。