基因导入气道。
Gene delivery to the airway.
作者信息
Keiser Nicholas W, Engelhardt John F
机构信息
University of Iowa, Iowa City, Iowa, USA.
出版信息
Curr Protoc Hum Genet. 2013 Jul;Chapter 13:Unit 13.9. doi: 10.1002/0471142905.hg1309s78.
Abstract
This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of tracheal xenografts, as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.
摘要
本单元描述了几种常用气道模型的构建及基因导入方法。首先介绍了原代气道上皮细胞的分离和转导。接下来，概述了极化气道上皮单层的制备方法。还描述了这些极化细胞的转导过程。介绍了气管异种移植的构建方法，以及对这些异种移植进行离体和体内基因转移的方法。最后，包括了一种向啮齿动物肺部进行体内基因递送的方法。支持方案中给出了评估转基因表达的方法。
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