Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455 USA.
IEEE Trans Biomed Circuits Syst. 2012 Jun;6(3):189-96. doi: 10.1109/TBCAS.2011.2172992.
We present a label-free CMOS field-effect transistor sensing array to detect the surface potential change affected by the negative charge in DNA molecules for real-time monitoring and quantification. The proposed CMOS bio sensor includes a new sensing pixel architecture implemented with correlated double sampling for reducing offset fixed pattern noise and 1/f noise of the sensing devices. We incorporated non-surface binding detection which allows real-time continuous monitoring of DNA concentrations without immobilizing them on the sensing surface. Various concentrations of 19-bp oligonucleotides solution can be discriminated using the prototype device fabricated in 1- μm double-poly double-metal standard CMOS process. The detection limit was measured as 1.1 ng/μl with a dynamic range of 40 dB and the transient response time was measured less than 20 seconds.
我们展示了一种无标记的互补金属氧化物半导体（CMOS）场效应晶体管传感阵列，用于检测受DNA分子中负电荷影响的表面电位变化，以进行实时监测和定量分析。所提出的CMOS生物传感器包括一种新的传感像素架构，该架构采用相关双采样实现，以减少传感设备的失调固定模式噪声和1/f噪声。我们采用了非表面结合检测方法，无需将DNA固定在传感表面就能对其浓度进行实时连续监测。使用采用1-μm双多晶硅双金属标准CMOS工艺制造的原型设备，可以区分不同浓度的19碱基对寡核苷酸溶液。检测限测得为1.1 ng/μl，动态范围为40 dB，瞬态响应时间测得小于20秒。
