Kirch D G, Taylor T R, Gerhardt G A, Benowitz N L, Stephen C, Wyatt R J
Neuropsychiatry Branch, National Institute of Mental Health, Neuroscience Center, Saint Elizabeths, Washington, D.C. 20032.
Neuropharmacology. 1990 Jun;29(6):599-602. doi: 10.1016/0028-3908(90)90073-z.
Caffeine was chronically administered in four doses (0, 10, 25, and 50 mg/kg/day) to rats via twice-daily intraperitoneal injections for 30 days. Concentrations of brain tissue monoamines, dopamine (DA), norepinephrine (NE), and serotonin (5HT), and monoamine metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG), and 5-hydroxyindoleacetic acid (5HIAA), were determined. At the 10 mg/kg/day dose, no significant changes were found compared with controls. At 25 mg/kg/day and 50 mg/kg/day significant changes were observed within each monoamine system. In striatum, DA and 5HT were increased, while DOPAC was decreased. In frontal cortex, NE was increased. In cerebellum, 5HT and MHPG were increased.
通过每日两次腹腔注射，以四种剂量（0、10、25和50毫克/千克/天）对大鼠长期给予咖啡因，持续30天。测定脑组织中单胺、多巴胺（DA）、去甲肾上腺素（NE）和5-羟色胺（5HT）的浓度，以及单胺代谢产物二羟基苯乙酸（DOPAC）、高香草酸（HVA）、3-甲氧基-4-羟基苯乙二醇（MHPG）和5-羟基吲哚乙酸（5HIAA）的浓度。在10毫克/千克/天的剂量下，与对照组相比未发现显著变化。在25毫克/千克/天和50毫克/千克/天的剂量下，每个单胺系统内均观察到显著变化。在纹状体中，DA和5HT增加，而DOPAC减少。在额叶皮质中，NE增加。在小脑中，5HT和MHPG增加。
