Zhang Tao, Yang Zu-Jun
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Yi Chuan. 2013 Jul;35(7):867-74. doi: 10.3724/sp.j.1005.2013.00867.
Eukaryotes's gene expression and regulation relies on the interaction of their cis-acting elements and trans-acting factors. The cis-elements are specific DNA sequences in the genome and frequently located in the untranslated regions. The trans-acting factors are usually considered to be proteins that bind to the cis-acting sequences to regulate gene expression. It is well known that the cis-elements are always associated with DNase I hypersensitive site, which is a signature of open chromatin. The identification of all the functional cis-element using high-throughput method in plant genome has not been initiated in plant genomes. With the rapid achievement of genomics studies, an increasing number of plant genomes have been sequenced. Genome-wide identification of DNase I hypersensitive sites will be a considerably efficient method to locate cis-element in plants, which will provide a vital potential for further plant functional genomics. The present review is to reveal the recent progresses on identification and analysis of DNase I hypersensitive site in plant genomes.
真核生物的基因表达和调控依赖于其顺式作用元件和反式作用因子之间的相互作用。顺式元件是基因组中的特定DNA序列，通常位于非翻译区。反式作用因子通常被认为是与顺式作用序列结合以调控基因表达的蛋白质。众所周知，顺式元件总是与DNase I超敏感位点相关联，该位点是开放染色质的标志。在植物基因组中，尚未开始使用高通量方法鉴定所有功能性顺式元件。随着基因组学研究的迅速发展，越来越多的植物基因组已被测序。全基因组鉴定DNase I超敏感位点将是在植物中定位顺式元件的一种相当有效的方法，这将为进一步的植物功能基因组学提供重要潜力。本综述旨在揭示植物基因组中DNase I超敏感位点鉴定和分析的最新进展。
