Suppr超能文献

调控白色-烟色形态转换的转录调控网络结构。

Structure of the transcriptional network controlling white-opaque switching in Candida albicans.

机构信息

Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA, 94158, USA.

出版信息

Mol Microbiol. 2013 Oct;90(1):22-35. doi: 10.1111/mmi.12329. Epub 2013 Aug 25.

Abstract

The human fungal pathogen Candida albicans can switch between two phenotypic cell types, termed 'white' and 'opaque'. Both cell types are heritable for many generations, and the switch between the two types occurs epigenetically, that is, without a change in the primary DNA sequence of the genome. Previous work identified six key transcriptional regulators important for white-opaque switching: Wor1, Wor2, Wor3, Czf1, Efg1, and Ahr1. In this work, we describe the structure of the transcriptional network that specifies the white and opaque cell types and governs the ability to switch between them. In particular, we use a combination of genome-wide chromatin immunoprecipitation, gene expression profiling, and microfluidics-based DNA binding experiments to determine the direct and indirect regulatory interactions that form the switch network. The six regulators are arranged together in a complex, interlocking network with many seemingly redundant and overlapping connections. We propose that the structure (or topology) of this network is responsible for the epigenetic maintenance of the white and opaque states, the switching between them, and the specialized properties of each state.

摘要

人类真菌病原体白色念珠菌可以在两种表型细胞类型之间切换,分别称为“白”和“不透明”。这两种细胞类型都可以遗传多代,而且两种类型之间的转换是表观遗传的,即基因组的主要 DNA 序列没有变化。以前的工作确定了六个关键的转录调节因子,它们对白-不透明转换很重要: Wor1、Wor2、Wor3、Czf1、Efg1 和 Ahr1。在这项工作中,我们描述了指定白和不透明细胞类型并控制它们之间转换能力的转录网络的结构。特别是,我们使用全基因组染色质免疫沉淀、基因表达谱和基于微流控的 DNA 结合实验相结合的方法,确定了形成开关网络的直接和间接调节相互作用。这六个调节剂排列在一起,形成一个复杂的、相互关联的网络,具有许多看似冗余和重叠的连接。我们提出,该网络的结构(或拓扑结构)负责白色和不透明状态的表观遗传维持、它们之间的转换以及每种状态的特殊性质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d29c/3888361/b332bb898bd9/nihms508843f1.jpg

相似文献

引用本文的文献

5
Is Cryptococcus neoformans a pleomorphic fungus?新型隐球菌是多形性真菌吗?
Curr Opin Microbiol. 2024 Dec;82:102539. doi: 10.1016/j.mib.2024.102539. Epub 2024 Sep 10.
6
White-opaque switching in : cell biology, regulation, and function.: 细胞生物学、调控与功能中的白-不透明态转变。
Microbiol Mol Biol Rev. 2024 Jun 27;88(2):e0004322. doi: 10.1128/mmbr.00043-22. Epub 2024 Mar 28.
7
Physiological adventures in : farnesol and ubiquinones.生理学奇遇:法呢醇和泛醌。
Microbiol Mol Biol Rev. 2024 Mar 27;88(1):e0008122. doi: 10.1128/mmbr.00081-22. Epub 2024 Mar 4.
10
Inferring gene regulatory networks using transcriptional profiles as dynamical attractors.利用转录谱作为动态吸引子推断基因调控网络。
PLoS Comput Biol. 2023 Aug 22;19(8):e1010991. doi: 10.1371/journal.pcbi.1010991. eCollection 2023 Aug.

本文引用的文献

8
Target specificity of the Candida albicans Efg1 regulator.白色念珠菌 Efg1 调节因子的靶标特异性。
Mol Microbiol. 2011 Nov;82(3):602-18. doi: 10.1111/j.1365-2958.2011.07837.x. Epub 2011 Oct 10.
9
Control of the embryonic stem cell state.胚胎干细胞状态的控制。
Cell. 2011 Mar 18;144(6):940-54. doi: 10.1016/j.cell.2011.01.032.

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验