Nobata Tadatoshi, Kyougoku Hirohisa, Miyano Takashi
Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
J Reprod Dev. 2013 Oct;59(5):500-5. doi: 10.1262/jrd.2012-182. Epub 2013 Jul 15.
During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whole-mount preparations to visualize the nucleoli. Nucleoli of growing and full-grown oocytes were stained blue with hematoxylin. Using this staining method, the changes in the oocyte nucleolus during maturation were examined. The nucleolar diameter gradually decreased in maturing oocytes (10.7 ± 0.1 μm to 9.0 ± 0.7 μm, P<0.05) before germinal vesicle breakdown (GVBD). The results suggest that the nucleolar volume of oocytes decreases before GVBD.
在卵母细胞生长过程中，核仁的形态会转变为致密且均匀的结构。完全成熟的卵母细胞中的致密核仁不会被醋酸洋红染色或免疫荧光染色所标记。在本研究中，我们开发了一种苏木精染色方法，用于对整装制片中的猪卵母细胞进行核仁可视化。生长中和完全成熟的卵母细胞的核仁被苏木精染成蓝色。使用这种染色方法，研究了卵母细胞成熟过程中核仁的变化。在生发泡破裂（GVBD）之前，成熟卵母细胞中的核仁直径逐渐减小（从10.7±0.1μm降至9.0±0.7μm，P<0.05）。结果表明，卵母细胞的核仁体积在GVBD之前会减小。
