Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), 08003 Barcelona, Spain.
J Cell Biol. 2013 Jul 22;202(2):241-50. doi: 10.1083/jcb.201303163. Epub 2013 Jul 15.
Here we report that the kinesin-5 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for protein transport from the Golgi complex to the cell surface in Drosophila S2 cells. Disrupting the function of its mammalian orthologue, Eg5, in HeLa cells inhibited secretion of a protein called pancreatic adenocarcinoma up-regulated factor (PAUF) but, surprisingly, not the trafficking of vesicular stomatitis virus G protein (VSV-G) to the cell surface. We have previously reported that PAUF is transported from the trans-Golgi network (TGN) to the cell surface in specific carriers called CARTS that exclude VSV-G. Inhibition of Eg5 function did not affect the biogenesis of CARTS; however, their migration was delayed and they accumulated near the Golgi complex. Altogether, our findings reveal a surprising new role of Eg5 in nonmitotic cells in the facilitation of the transport of specific carriers, CARTS, from the TGN to the cell surface.
在这里，我们报告说，驱动蛋白-5 马达 Klp61F 在有丝分裂中双极纺锤体的形成中起作用，它是果蝇 S2 细胞中从高尔基体复合物向细胞表面运输蛋白质所必需的。破坏其哺乳动物同源物 Eg5 在 HeLa 细胞中的功能抑制了一种称为胰腺腺癌上调因子 (PAUF) 的蛋白质的分泌，但令人惊讶的是，它并没有抑制囊泡性口炎病毒 G 蛋白 (VSV-G) 向细胞表面的运输。我们之前曾报道过，PAUF 是从高尔基体网络 (TGN) 运输到细胞表面的特定载体 CARTS 中，这些载体排除了 VSV-G。Eg5 功能的抑制并不影响 CARTS 的生物发生;然而，它们的迁移被延迟，并且它们在高尔基体附近积累。总之，我们的发现揭示了 Eg5 在非有丝分裂细胞中促进特定载体 CARTS 从 TGN 向细胞表面运输的一个令人惊讶的新作用。
