Meydan Sedat, Nacar Ahmet, Oztürk Hasan Oktay, Tas Ufuk, Köse Evren, Zararsiz Ismail, Yılmaz Nigar, Kus Ilter
Department of Anatomy, Medical Faculty, Bezmialem Vakif University, İstanbul, Turkey
Department of Histology-Embryology, Tayfur Ata Sokmen Medical Faculty, Mustafa Kemal University, Hatay, Turkey.
Toxicol Ind Health. 2016 Jan;32(1):15-21. doi: 10.1177/0748233713485890. Epub 2013 Jul 15.
Caffeic acid phenethyl ester (CAPE) has antioxidant and anti-inflammatory properties. The aim of this study is to examine the negative effects of toluene on kidney tissues and functions and to investigate the protective effects of CAPE against toluene-induced nephrotoxicity in rats. A total of 21 male Wistar rats were divided into three groups of equal number in each. The rats in group I were the controls. Toluene was intraperitoneally injected into the rats in group II with a dose of 500 mg/kg. Rats in group III received CAPE daily while exposed to toluene. After 14 days of experimental period, all rats were killed by decapitation. Enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) and malondialdehyde (MDA) levels were studied in the rat kidneys. Blood urea nitrogen (BUN) and serum creatinine levels were measured for renal function. The CAT and SOD enzyme activities and serum creatinine levels were significantly increased in rats treated with toluene when compared with the controls. But GSH-Px activity, MDA, and BUN levels showed statistically nonsignificant changes. However, increased CAT and SOD enzyme activities and decreased serum creatinine levels were detected in the rats that received CAPE while exposed to toluene. The GSH-Px activity and MDA and BUN levels in the same group did not show statistically significant changes. The results of our study demonstrated that toluene damages kidney tissue and is a nephrotoxic substance. CAPE was able to prevent the renal damage as antioxidant, antitoxic, and nephroprotective agent.
咖啡酸苯乙酯（CAPE）具有抗氧化和抗炎特性。本研究的目的是检测甲苯对大鼠肾脏组织和功能的负面影响，并研究CAPE对甲苯诱导的大鼠肾毒性的保护作用。总共21只雄性Wistar大鼠被平均分为三组。第一组大鼠为对照组。第二组大鼠腹腔注射剂量为500 mg/kg的甲苯。第三组大鼠在接触甲苯的同时每日接受CAPE。实验期14天后，所有大鼠断头处死。研究大鼠肾脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的酶活性以及丙二醛（MDA）水平。测量血尿素氮（BUN）和血清肌酐水平以评估肾功能。与对照组相比，甲苯处理的大鼠中CAT和SOD酶活性以及血清肌酐水平显著升高。但GSH-Px活性、MDA和BUN水平显示出无统计学意义的变化。然而，在接触甲苯的同时接受CAPE的大鼠中，检测到CAT和SOD酶活性增加以及血清肌酐水平降低。同一组中的GSH-Px活性、MDA和BUN水平未显示出统计学意义的变化。我们的研究结果表明，甲苯会损害肾脏组织，是一种肾毒性物质。CAPE作为抗氧化、抗毒性和肾保护剂能够预防肾脏损伤。
