Korea Electrotechnology Research Institute, 111 Hanggaul-ro, Ansan, 426-170, Republic of Korea.
J Biomed Nanotechnol. 2013 Jun;9(6):1060-4. doi: 10.1166/jbn.2013.1512.
We report on the enhancement of sensitivity of SPR biosensor by modifying the metal surface. A mixture layer, in which gold and dielectric medium coexist, was simply prepared by increasing the roughness of gold surface deposited onto a glass substrate via the reduction reaction. The absorbance spectrum and atomic force microscopy study showed that the average roughness of modified substrate increased by 4 times, compared to the unmodified substrate. The SPR curves of the unmodified substrate and the modified substrate were measured with the various refractive index of the dielectric medium. The sensitivity of modified substrate to the refractive index of dielectric medium was increased by 1.5 times, compared to the unmodified substrate. In result, the addition of the mixture layer between the gold surface and the dielectric medium by the reduction reaction can provides the enhancement of sensitivity in the SPR biosensor.
我们报告了通过修饰金属表面来提高 SPR 生物传感器灵敏度的方法。通过增加沉积在玻璃基底上的金表面的粗糙度，通过还原反应简单地制备了金和介电介质共存的混合层。吸收光谱和原子力显微镜研究表明，与未修饰的基底相比，修饰后的基底的平均粗糙度增加了 4 倍。使用不同介电介质的折射率测量了未修饰基底和修饰基底的 SPR 曲线。与未修饰的基底相比，修饰后的基底对介电介质的折射率的灵敏度提高了 1.5 倍。结果表明，通过还原反应在金表面和介电介质之间添加混合层可以提高 SPR 生物传感器的灵敏度。
