School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China.
Biochem Cell Biol. 2013 Aug;91(4):244-51. doi: 10.1139/bcb-2013-0002. Epub 2013 Mar 28.
Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 μg/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of β1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.
中华真地鳖是一种重要的药用昆虫。本研究采用硫酸铵沉淀、超滤、CM Sepharose Fast Flow 阳离子交换、Q Sepharose High Performance (HP)阴离子交换、Butyl Sepharose HP 疏水层析和 Superdex 75 凝胶过滤层析等技术，从该物种中纯化得到一种 72 kDa 的抗癌蛋白，命名为 EPS72。EPS72 对人肺癌 A549 细胞系表现出很强的抗癌活性（IC50，18.76μg/mL）。进一步的研究表明，EPS72 可诱导 A549 细胞脱落和凋亡，抑制细胞与纤维连接蛋白和胶原 IV 的黏附，并抑制细胞迁移和侵袭。此外，EPS72 显著降低了β1-整合素的表达。本研究提示，由于其可能的抗转移活性，EPS72 可能有潜力被开发为一种新型的抗癌治疗药物。
