School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
J Control Release. 2013 Oct 28;171(2):113-21. doi: 10.1016/j.jconrel.2013.07.006. Epub 2013 Jul 13.
A dual-function nano-system for synergistic photodynamic therapy (PDT) and photothermal therapy (PTT) was constructed. Gold nanorods (GNRs) as a PTT agent and chlorin e6 (Ce6) as a photosensitizer (PS) for PDT were loaded into a chitosan-functionalized, Pluronic-based nanogel that was proven to be an efficient delivery vehicle to the tumor site in vivo. Previously reported combined therapy systems relied on quenching and de-quenching of PS for PDT upon thermo-impact of PTT, thus only PTT followed by PDT procedure was possible. In contrast, the present dual-acting system has no quenching between PS and GNRs by preventing direct contact and self-aggregation of photo-sensitizers, allowing independent PDT or PTT procedure. In both in vitro cell culture and in vivo tumor-bearing mice experiments, a remarkably enhanced tumor ablation compared to the treatment of PDT or PTT only was observed by the treatment of PDT followed by PTT, but not significantly by the treatment of PTT followed by PDT. Thus, the present study demonstrated the synergistic effect of PDT and PTT in a sequence-dependent manner, and our system is a promising dual function nano system to achieve the enhanced phototherapy in vivo.
构建了一种用于协同光动力疗法（PDT）和光热疗法（PTT）的双功能纳米系统。金纳米棒（GNRs）作为 PTT 试剂和氯卟啉 e6（Ce6）作为 PDT 的光敏剂（PS）被装载到壳聚糖功能化的基于 Pluronic 的纳米凝胶中，该纳米凝胶被证明是体内肿瘤部位的有效递送载体。以前报道的联合治疗系统依赖于 PTT 的热影响下 PS 的猝灭和去猝灭，从而仅可能进行 PTT 后 PDT 程序。相比之下，当前的双作用系统通过防止 PS 和 GNRs 的直接接触和自聚集来防止 PS 和 GNRs 之间的猝灭，从而允许独立的 PDT 或 PTT 程序。在体外细胞培养和体内荷瘤小鼠实验中，与仅 PDT 或 PTT 治疗相比，通过 PDT 后 PTT 治疗观察到显著增强的肿瘤消融，而通过 PTT 后 PDT 治疗则没有显著增强。因此，本研究以序列依赖的方式证明了 PDT 和 PTT 的协同效应，并且我们的系统是一种有前途的双功能纳米系统，可在体内实现增强的光疗。
