Plant Protection Laboratory of National Institute of Agricultural Research of Tunisia, Ariana, Tunisia.
Biomed Res Int. 2013;2013:838145. doi: 10.1155/2013/838145. Epub 2013 Jun 15.
The natural occurrence of entomophthoralean fungi pathogenic towards aphids on cereal and potato crops was investigated in the years 2009, 2010, and 2011. Infected aphids were sampled in three bioclimatic zones in Tunisia (Beja, Cap bon, and Kairouan) and fungal species were determined based on morphological characters such as shape, size, and number of nuclei in the primary conidia. Polymerase Chain Reaction (PCR) on the internal transcribed spacer 1 region (ITS1) was used to verify morphological determination. Both methods gave consistent results and we documented for the first time the natural occurrence of two fungal species from the order Entomophthorales (phylum Entomophthoromycota), Pandora neoaphidis and Entomophthora planchoniana. Both fungi were recorded on the aphid species Sitobion avenae and Myzus persicae on barley ears and potato leaves, respectively. Moreover, natural mixed infections by both species (P. neoaphidis and E. planchoniana) were documented on the target aphids. This investigation provides basic information of entomopathogenic fungi infecting economically important aphids in Tunisia.
对 2009 年、2010 年和 2011 年在突尼斯三个生物气候带（贝贾、卡比托和凯鲁万）的谷物和马铃薯作物上的具有致病性的昆虫真菌自然发生情况进行了调查。从受感染的蚜虫中取样，并根据初级分生孢子的形状、大小和细胞核数量等形态特征确定真菌种类。聚合酶链反应（PCR）在内部转录间隔区 1 区域（ITS1）上进行，以验证形态学鉴定。这两种方法都得到了一致的结果，我们首次记录了来自昆虫纲（昆虫真菌门）的两种真菌，即新粘虫 Pandora neoaphidis 和粘虫 Entomophthora planchoniana 的自然发生。这两种真菌分别在大麦穗上的麦长管蚜和马铃薯叶上的桃蚜上被记录。此外，还记录了目标蚜虫上两种真菌（P. neoaphidis 和 E. planchoniana）的自然混合感染。这项调查为突尼斯感染经济上重要的蚜虫的昆虫病原真菌提供了基本信息。
