猪小肠黏膜细胞中β-半乳糖苷酶的细胞内分布

Intracellular distribution of beta-galactosidases in mucosal cells from hog small intestine.

Sato M, Yamashina I

Biochim Biophys Acta. 1975 Jul 27;397(1):179-87. doi: 10.1016/0005-2744(75)90191-6.

Abstract

Intracellular distribution of three types of beta-galactosidases (beta-D-galactoside galactohydrolase EC 3.2.1.12) i.e. hetero beta-galactosidase, lactase and acid beta-galactosidase, was studied by examining the properties of subcellular fractions isolated by a systematic fractionation of mucosal cells of the small intestine of the hog. Localization of hetero beta-galactosidase in cytosol could be shown. 2. Localization of lactase in the brush borders was shown by analyzing the purified brush borders prepared separately. 3. To domonstrate the lysosomal localization of acid belta-galactosidase, lysosomes were purified separately and their extract was chromatographed on a hydroxylapatite column. The activities of various enzymes in the purified lysosomes as well as in the intermediary fractions obtained during lysosome purification and the pattern of the hydroxylapatite chromatography led us to conclude that acid beta-galactosidase is a lysosomal enzyme.

摘要

通过研究对猪小肠黏膜细胞进行系统分级分离得到的亚细胞组分的特性，对三种β-半乳糖苷酶（β-D-半乳糖苷半乳糖水解酶，EC 3.2.1.12），即杂合β-半乳糖苷酶、乳糖酶和酸性β-半乳糖苷酶的细胞内分布进行了研究。结果表明杂合β-半乳糖苷酶定位于胞质溶胶中。2. 通过分别分析纯化得到的刷状缘，显示了乳糖酶在刷状缘中的定位。3. 为了证明酸性β-半乳糖苷酶的溶酶体定位，分别纯化了溶酶体，并将其提取物在羟磷灰石柱上进行色谱分析。纯化溶酶体以及溶酶体纯化过程中获得的中间组分中各种酶的活性，以及羟磷灰石色谱图谱，使我们得出结论，酸性β-半乳糖苷酶是一种溶酶体酶。