大鼠小肠β-半乳糖苷酶。通过离子交换色谱法和凝胶过滤法进行分离。
Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.
作者信息
Asp N G, Dahlqvist A
出版信息
Biochem J. 1968 Feb;106(4):841-5. doi: 10.1042/bj1060841.
Abstract
- The chromatography of rat small-intestinal beta-galactosidase activities on gel-filtration and ion-exchange columns has been studied. Five different substrates were used to measure beta-galactosidase activity (lactose, phenyl beta-galactoside, o-nitrophenyl beta-galactoside, p-nitrophenyl beta-galactoside and 6-bromo-2-naphthyl beta-galactoside) and the activity was measured at one acid and one more neutral pH value. 2. By gel filtration one acid beta-galactosidase, hydrolysing lactose and the hetero-beta-galactosides at about the same rate, and one more neutral beta-galactosidase, hydrolysing lactose much more rapidly than the hetero-beta-galactosides, were separated. 3. By ion-exchange chromatography the acid enzyme was fractionated into two components. These may be individual enzymes or different forms of the same enzyme.
摘要
- 对大鼠小肠β-半乳糖苷酶活性在凝胶过滤柱和离子交换柱上的色谱情况进行了研究。使用了五种不同的底物来测定β-半乳糖苷酶活性（乳糖、苯基β-半乳糖苷、邻硝基苯基β-半乳糖苷、对硝基苯基β-半乳糖苷和6-溴-2-萘基β-半乳糖苷），并且在一种酸性pH值和另一种接近中性的pH值下测定活性。2. 通过凝胶过滤，分离出一种酸性β-半乳糖苷酶，它以大致相同的速率水解乳糖和杂β-半乳糖苷，以及另一种接近中性的β-半乳糖苷酶，其水解乳糖的速度比杂β-半乳糖苷快得多。3. 通过离子交换色谱法，酸性酶被分离成两个组分。这些可能是单独的酶，或者是同一酶的不同形式。
相似文献
1
Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.大鼠小肠β-半乳糖苷酶。通过离子交换色谱法和凝胶过滤法进行分离。
Biochem J. 1968 Feb;106(4):841-5. doi: 10.1042/bj1060841.
2
Rat small-intestinal beta-galactosidases. Kinetic studies with three separated fractions.大鼠小肠β-半乳糖苷酶。对三个分离组分的动力学研究。
Biochem J. 1968 Nov;110(1):143-50. doi: 10.1042/bj1100143.
3
Rat small-intestinal beta-galactosidases. Influence of pH on the hydrolysis of different substrates.大鼠小肠β-半乳糖苷酶。pH对不同底物水解的影响。
Biochem J. 1967 Apr;103(1):86-9. doi: 10.1042/bj1030086.
4
Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase.人类小肠β-半乳糖苷酶。一种乳糖酶和一种杂合β-半乳糖苷酶的分离与特性分析。
Biochem J. 1969 Sep;114(2):351-9. doi: 10.1042/bj1140351.
5
Intestinal beta-galactosidases. I. Separation and characterization of three enzymes in normal human intestine.肠道β-半乳糖苷酶。I. 正常人肠道中三种酶的分离与特性鉴定。
J Clin Invest. 1969 Apr;48(4):716-28. doi: 10.1172/JCI106029.
6
Human small-intestinal -galactosidases. Separation and characterization of three forms of an acid -galactosidase.人小肠β-半乳糖苷酶。一种酸性β-半乳糖苷酶三种形式的分离与特性鉴定
Biochem J. 1971 Jan;121(2):299-308. doi: 10.1042/bj1210299.
7
Rat small-intestinal beta-galactosidases. Studies on the fractionation of "acid" beta-galactosidase with isoelectric focusing, gel filtration and ion-exchange chromatography.大鼠小肠β-半乳糖苷酶。关于用等电聚焦、凝胶过滤和离子交换色谱法分离“酸性”β-半乳糖苷酶的研究。
Biochem J. 1970 Apr;117(2):369-75. doi: 10.1042/bj1170369.
8
Small intestinal beta-galactosidase activity in the horse.马的小肠β-半乳糖苷酶活性
Gut. 1973 Jul;14(7):535-40. doi: 10.1136/gut.14.7.535.
9
Intracellular distribution of beta-galactosidases in mucosal cells from hog small intestine.猪小肠黏膜细胞中β-半乳糖苷酶的细胞内分布
Biochim Biophys Acta. 1975 Jul 27;397(1):179-87. doi: 10.1016/0005-2744(75)90191-6.
10
Intestinal beta-galactosidases in adult low lactase activity and in congenital lactase deficiency.成人低乳糖酶活性和先天性乳糖酶缺乏症中的肠道β-半乳糖苷酶
Enzyme. 1974;18(1):84-102. doi: 10.1159/000459416.
引用本文的文献
1
Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase.人类小肠β-半乳糖苷酶。一种乳糖酶和一种杂合β-半乳糖苷酶的分离与特性分析。
Biochem J. 1969 Sep;114(2):351-9. doi: 10.1042/bj1140351.
2
Characteristics of beta-galactosidase in the mucosa of the small intestine of infant rats. Physicochemical properties.幼鼠小肠黏膜中β-半乳糖苷酶的特性。物理化学性质。
Biochem J. 1969 Sep;114(3):621-7. doi: 10.1042/bj1140621.
3
Intestinal disaccharidase activities in the chick.雏鸡肠道双糖酶活性
Biochem J. 1969 Mar;112(1):51-9. doi: 10.1042/bj1120051.
4
Rat small-intestinal beta-galactosidases. Kinetic studies with three separated fractions.大鼠小肠β-半乳糖苷酶。对三个分离组分的动力学研究。
Biochem J. 1968 Nov;110(1):143-50. doi: 10.1042/bj1100143.
5
Multiple forms of pig intestinal beta-galactosidase and related activities.猪肠道β-半乳糖苷酶的多种形式及相关活性
Biochem J. 1970 Sep;119(3):8P. doi: 10.1042/bj1190008pa.
6
Rat small-intestinal beta-galactosidases. Studies on the fractionation of "acid" beta-galactosidase with isoelectric focusing, gel filtration and ion-exchange chromatography.大鼠小肠β-半乳糖苷酶。关于用等电聚焦、凝胶过滤和离子交换色谱法分离“酸性”β-半乳糖苷酶的研究。
Biochem J. 1970 Apr;117(2):369-75. doi: 10.1042/bj1170369.
7
Effect of cortisone on the developmental pattern of the neutral and the acid beta-galactosidase of the small intestine of the rat.可的松对大鼠小肠中性和酸性β-半乳糖苷酶发育模式的影响。
Biochem J. 1970 Apr;117(3):467-71. doi: 10.1042/bj1170467.
8
Human small-intestinal -galactosidases. Separation and characterization of three forms of an acid -galactosidase.人小肠β-半乳糖苷酶。一种酸性β-半乳糖苷酶三种形式的分离与特性鉴定
Biochem J. 1971 Jan;121(2):299-308. doi: 10.1042/bj1210299.
9
Gargoylism: hydrolysis of beta-galactosides and tissure accumulation of galactose- and mannose-containing compounds.承雷氏病：β-半乳糖苷水解以及含半乳糖和甘露糖化合物的组织蓄积。
J Clin Invest. 1970 Feb;49(2):216-24. doi: 10.1172/JCI106230.
10
Small intestinal beta-galactosidase activity in the horse.马的小肠β-半乳糖苷酶活性
Gut. 1973 Jul;14(7):535-40. doi: 10.1136/gut.14.7.535.
本文引用的文献
1
Specificity and multiple forms of beta-galactosidase in the rat.大鼠体内β-半乳糖苷酶的特异性及多种形式
Biochem J. 1965 Oct;97(1):59-66. doi: 10.1042/bj0970059.
2
RAT INTESTINAL 6-BROMO-2-NAPHTHYL GLYCOSIDASE AND DISACCHARIDASE ACTIVITIES. II. SOLUBILIZATION AND SEPARATION OF THE SMALL-INTESTINAL ENZYMES.大鼠肠道6-溴-2-萘基糖苷酶和双糖酶活性。II. 小肠酶的溶解与分离
Arch Biochem Biophys. 1965 Jan;109:159-67. doi: 10.1016/0003-9861(65)90301-2.
3
METHOD FOR ASSAY OF INTESTINAL DISACCHARIDASES.肠道双糖酶的测定方法。
Anal Biochem. 1964 Jan;7:18-25. doi: 10.1016/0003-2697(64)90115-0.
4
Studies of small intestine during development. I. Distribution and activity of beta-galactosidase.发育过程中小肠的研究。I. β-半乳糖苷酶的分布与活性。
Biochim Biophys Acta. 1962 Aug 13;62:353-62. doi: 10.1016/0006-3002(62)90097-5.
5
Determination of maltase and isomaltase activities with a glucose-oxidase reagent.用葡萄糖氧化酶试剂测定麦芽糖酶和异麦芽糖酶活性。
Biochem J. 1961 Sep;80(3):547-51. doi: 10.1042/bj0800547.
6
Rat small-intestinal beta-galactosidases. Influence of pH on the hydrolysis of different substrates.大鼠小肠β-半乳糖苷酶。pH对不同底物水解的影响。
Biochem J. 1967 Apr;103(1):86-9. doi: 10.1042/bj1030086.
7
Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.大鼠肾脏中β-半乳糖苷酶、β-葡萄糖苷酶、β-葡萄糖醛酸酶和N-乙酰-β-葡萄糖胺酶的分离及性质
Biochem J. 1967 Feb;102(2):525-32. doi: 10.1042/bj1020525.
8
Beta-galactosidase activity in human intestinal lactases.人肠道乳糖酶中的β-半乳糖苷酶活性
Biochim Biophys Acta. 1966 Feb 14;113(2):390-3. doi: 10.1016/s0926-6593(66)80077-2.
9
Activity of beta-galactosidase in homogenates and isolated microvilli fraction of jejunal mucosa from suckling rats.哺乳期大鼠空肠黏膜匀浆及分离的微绒毛部分中β-半乳糖苷酶的活性。
Biochem J. 1965 Aug;96(2):492-4. doi: 10.1042/bj0960492.
Zotero 插件