Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
J Control Release. 2013 Oct 28;171(2):178-83. doi: 10.1016/j.jconrel.2013.07.003. Epub 2013 Jul 14.
Transdermal delivery is a useful and attractive method for drug delivery, even though the stratum corneum is a major barrier of protein translocation into the skin. To achieve protein delivery through the stratum corneum, we first cast gold nanorods, acting as a heating device in response to near-infrared light irradiation, onto the skin surface. After applying an aqueous solution of ovalbumin to the skin, the skin was irradiated by near-infrared laser light. Irradiation of the skin using a continuous-wave laser increased the skin temperature resulting in an efficient translocation of ovalbumin into the skin. Furthermore, migration of inflammation cells and induction heat shock protein 70 (HSP70) were observed. Irradiation of the skin using a pulsed laser caused an enhanced permeability of the stratum corneum without an increase in skin temperature, migration of inflammation cells, or HSP70 induction. This effect is due to the pulsed-laser irradiation increasing the temperature of a limited part of the skin surface. Thus, the physiological response of skin is dependent on the type of laser light used. It is anticipated that this phenomenon will find wide application in such applications as, for example, general transdermal protein delivery and transdermal vaccination.
经皮给药是一种有用且有吸引力的药物传递方法，尽管角质层是蛋白质进入皮肤的主要屏障。为了实现蛋白质透过角质层的传递，我们首先将金纳米棒（作为对近红外光照射的加热装置）铸到皮肤表面上。将卵清蛋白的水溶液应用于皮肤后，用近红外激光照射皮肤。连续波激光照射皮肤会增加皮肤温度，从而有效地将卵清蛋白转移到皮肤中。此外，还观察到炎症细胞的迁移和热休克蛋白 70（HSP70）的诱导。使用脉冲激光照射皮肤会导致角质层的渗透性增强，而不会增加皮肤温度、炎症细胞的迁移或 HSP70 的诱导。这种效果是由于脉冲激光照射会增加皮肤表面有限部分的温度。因此，皮肤的生理反应取决于所用激光的类型。预计这种现象将在例如一般的经皮蛋白传递和经皮疫苗接种等应用中得到广泛应用。
