Shiiyama Rie, Fukushima Satoshi, Jinnin Masatoshi, Yamashita Junji, Miyashita Azusa, Nakahara Satoshi, Kogi Ai, Aoi Jun, Masuguchi Shinichi, Inoue Yuji, Ihn Hironobu
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
Melanoma Res. 2013 Oct;23(5):366-72. doi: 10.1097/CMR.0b013e328363e485.
Numerous studies have indicated that the serum levels of microRNAs are useful for the diagnosis or evaluation of activity in human diseases. However, determining the level of only one of the nearly 2000 microRNAs identified so far may be less significant. Accordingly, we examined the possibility that the expression pattern of multiple microRNAs in each patient may be a more reliable disease marker for melanoma, especially metastatic disease, focusing on the interaction among microRNAs. Six microRNAs (miR-9, miR-145, miR-150, miR-155, miR-203, and miR-205) were evaluated using real-time PCR in 11 patients with metastatic melanoma and in 16 patients without melanoma. The expression of the six microRNAs was significantly different between the patients with metastasis and those without it. MiR-9 and miR-205 and miR-203 and miR-205 showed significant correlations, and the combination of miR-9, miR-145, miR-150, miR-155, and miR-205 was more sensitive than when each miR was used individually to distinguish the patients with metastasis from those without it. This is the first report demonstrating the expression profiles of multiple microRNAs in melanoma patients. Clarifying the involvement of the microRNA network in the pathogenesis of melanoma may contribute to the development of new diagnostic tools and to advancing the understanding of this disease.
众多研究表明，微小RNA的血清水平可用于人类疾病的诊断或活动评估。然而，仅测定目前已鉴定出的近2000种微小RNA中的一种的水平，可能意义不大。因此，我们研究了每个患者中多种微小RNA的表达模式可能是黑色素瘤尤其是转移性疾病更可靠的疾病标志物的可能性，重点关注微小RNA之间的相互作用。使用实时聚合酶链反应对11例转移性黑色素瘤患者和16例无黑色素瘤患者的六种微小RNA（miR-9、miR-145、miR-150、miR-155、miR-203和miR-205）进行了评估。有转移的患者和无转移的患者之间六种微小RNA的表达存在显著差异。miR-9与miR-205以及miR-203与miR-205之间显示出显著相关性，并且miR-9、miR-145、miR-150、miR-155和miR-205的组合比单独使用每种miR区分有转移的患者和无转移的患者更敏感。这是第一份证明黑色素瘤患者中多种微小RNA表达谱的报告。阐明微小RNA网络在黑色素瘤发病机制中的作用可能有助于开发新的诊断工具，并增进对这种疾病的了解。
