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利用单克隆抗体对人肝细胞中 HCV 感染的进入因子 claudin-6 和 claudin-9 进行功能分析。

Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies.

机构信息

Inserm, U1110, Strasbourg, France.

出版信息

J Virol. 2013 Sep;87(18):10405-10. doi: 10.1128/JVI.01691-13. Epub 2013 Jul 17.

DOI:10.1128/JVI.01691-13
PMID:23864633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3753993/
Abstract

The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

摘要

Claudin-6 和 claudin-9 在丙型肝炎病毒 (HCV) 进入细胞中的相关性仍不清楚。我们制备了 claudin-6 或 claudin-9 特异性单克隆抗体，该抗体可抑制表达外源性 claudin-6 或 claudin-9 的非肝细胞系中的 HCV 进入。这些抗体对肝癌细胞或原代肝细胞中的 HCV 感染没有影响。因此，虽然 claudin-6 和 claudin-9 可作为细胞系中的进入因子，但 HCV 感染人肝细胞并不依赖于 claudin-6 和 claudin-9。

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本文引用的文献

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Isolate-dependent use of claudins for cell entry by hepatitis C virus.依赖于隔离蛋白的丙型肝炎病毒细胞进入。
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Identification of transferrin receptor 1 as a hepatitis C virus entry factor.鉴定转铁蛋白受体 1 为丙型肝炎病毒进入因子。
Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10777-82. doi: 10.1073/pnas.1301764110. Epub 2013 Jun 10.
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A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission.一种新型的单克隆抗 CD81 抗体通过遗传免疫产生，能有效抑制丙型肝炎病毒细胞间传播。
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The postbinding activity of scavenger receptor class B type I mediates initiation of hepatitis C virus infection and viral dissemination.清道夫受体 B 类 I 型的后结合活性介导丙型肝炎病毒感染和病毒传播的起始。
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Viral entry and escape from antibody-mediated neutralization influence hepatitis C virus reinfection in liver transplantation.病毒进入和逃避抗体介导的中和作用影响肝移植中丙型肝炎病毒的再感染。
J Exp Med. 2010 Aug 30;207(9):2019-31. doi: 10.1084/jem.20090766. Epub 2010 Aug 16.
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Monoclonal anti-claudin 1 antibodies prevent hepatitis C virus infection of primary human hepatocytes.单克隆抗紧密连接蛋白 1 抗体可预防丙型肝炎病毒感染原代人肝细胞。
Gastroenterology. 2010 Sep;139(3):953-64, 964.e1-4. doi: 10.1053/j.gastro.2010.05.073. Epub 2010 Jun 2.