Kuckelkorn U, Jacobasch G
Institute of Biochemistry, Humboldt University, Berlin, GDR.
Biomed Biochim Acta. 1990;49(2-3):S313-6.
An one-step-purification of native pyruvate kinase from human erythrocytes by immunoaffinity chromatography with monoclonal antibodies coupled on CNBr-activated Sepharose 4B is described. The recoveries of PK amounted up to 83%, with a 2400 fold purification of PK-R from human stroma free hemolysate.
本文描述了一种通过免疫亲和色谱法从人红细胞中一步纯化天然丙酮酸激酶的方法，该方法使用偶联在溴化氰活化的琼脂糖凝胶4B上的单克隆抗体。丙酮酸激酶的回收率高达83%，从无基质人溶血产物中对丙酮酸激酶-R进行了2400倍的纯化。
