Embrapa Soja, Londrina, Paraná, Brazil.
Hereditas. 2013 Jun;150(2-3):17-25. doi: 10.1111/j.1601-5223.2013.02275.x. Epub 2013 Jun 13.
Biological nitrogen fixation (BNF) is a key process, but despite the economic and environmental importance, few studies about quantitative trait loci (QTL) controlling BNF traits are available, even in the economically important crop soybean Glycine max (L.) Merr. In this study, a population of 157 F2:7 RILs derived from crossing soybean cultivars Bossier (high BNF capacity) and Embrapa 20 (medium BNF capacity) was genotyped with 105 simple sequence repeat markers (SSRs). The genetic map obtained has 1231.2 cM and covers about 50% of the genome, with an average interval of 18.1 cM. Three traits, nodule number (NN), the ratio nodule dry weight (NDW)/NN and shoot dry weight (SDW) were used to evaluate BNF performance. A composite interval mapping for multiple traits method (mCIM) analysis mapped two QTLs for SDW (LGs E and L), three for NN (LGs B1, E and I), and one for NDW/NN (LG I); all QTLs were of small effect (R-values ranging from 1.7% to 10.0%) and explained 15.4%, 13.8% and 6.5% of total variation for these three traits, respectively.
生物固氮（BNF）是一个关键过程，但尽管具有经济和环境重要性，关于控制 BNF 特性的数量性状位点（QTL）的研究很少，即使在经济上重要的作物大豆 Glycine max（L.）Merr 中也是如此。在这项研究中，通过杂交大豆品种 Bossier（高 BNF 能力）和 Embrapa 20（中 BNF 能力），产生了一个由 157 个 F2:7 RIL 组成的群体，用 105 个简单序列重复标记（SSR）进行了基因型分析。获得的遗传图谱长 1231.2 cM，覆盖了约 50%的基因组，平均间隔为 18.1 cM。三个特性，根瘤数（NN）、根瘤干重（NDW）/NN 比和地上部干重（SDW）用于评估 BNF 性能。对多个性状的复合区间作图（mCIM）分析在 LGs E 和 L 上定位了两个与 SDW 相关的 QTL，在 LGs B1、E 和 I 上定位了三个与 NN 相关的 QTL，在 LG I 上定位了一个与 NDW/NN 相关的 QTL；所有 QTL 的效应都较小（R 值范围为 1.7%至 10.0%），分别解释了这三个特性总变异的 15.4%、13.8%和 6.5%。
