Zhang W-K, Wang Y-J, Luo G-Z, Zhang J-S, He C-Y, Wu X-L, Gai J-Y, Chen S-Y
Plant Biotechnology Laboratory, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, 100101, Beijing, China.
Theor Appl Genet. 2004 Apr;108(6):1131-9. doi: 10.1007/s00122-003-1527-2. Epub 2004 Jan 22.
A set of 184 recombinant inbred lines (RILs) derived from soybean vars. Kefeng No.1 x Nannong 1138-2 was used to construct a genetic linkage map. The two parents exhibit contrasting characteristics for most of the traits that were mapped. Using restricted fragment length polymorphisms (RFLPs), simple sequence repeats (SSRs) and expressed sequence tags (ESTs), we mapped 452 markers onto 21 linkage groups and covered 3595.9 cM of the soybean genome. All of the linkage groups except linkage group F were consistent with those of the consensus map of Cregan et al. (1999). Linkage group F was divided into two linkage groups, F1 and F2. The map consisted of 189 RFLPs, 219 SSRs, 40 ESTs, three R gene loci and one phenotype marker. Ten agronomic traits-days to flowering, days to maturity, plant height, number of nodes on main stem, lodging, number of pods per node, protein content, oil content, 100-seed weight, and plot yield-were studied. Using winqtlcart, we detected 63 quantitative trait loci (QTLs) that had LOD>3 for nine of the agronomic traits (only exception being seed oil content) and mapped these on 12 linkage groups. Most of the QTLs were clustered, especially on groups B1 and C2. Some QTLs were mapped to the same loci. This pleiotropism was common for most of the QTLs, and one QTL could influence at most five traits. Seven EST markers were found to be linked closely with or located at the same loci as the QTLs. EST marker GmKF059a, encoding a repressor protein and mapped on group C2, accounted for about 20% of the total variation of days to flowering, plant height, lodging and nodes on the main stem, respectively.
以大豆品种科丰1号×南农1138 - 2衍生的184个重组自交系（RILs）构建遗传连锁图谱。双亲在大多数所定位的性状上表现出相反的特征。利用限制性片段长度多态性（RFLP）、简单序列重复（SSR）和表达序列标签（EST），我们将452个标记定位到21个连锁群上，覆盖大豆基因组3595.9 cM。除连锁群F外，所有连锁群均与Cregan等人（1999年）的整合图谱一致。连锁群F被分为两个连锁群，F1和F2。该图谱由189个RFLP、219个SSR、40个EST、3个R基因位点和1个表型标记组成。研究了10个农艺性状——开花天数、成熟天数、株高、主茎节数、倒伏性、每节荚数、蛋白质含量、油含量、百粒重和小区产量。使用winqtlcart，我们检测到63个数量性状位点（QTL），这些QTL在9个农艺性状上的LOD>3（种子油含量除外），并将它们定位到12个连锁群上。大多数QTL成簇分布，尤其是在B1和C2组。一些QTL被定位到相同的位点。这种多效性在大多数QTL中很常见，一个QTL最多可影响5个性状。发现7个EST标记与QTL紧密连锁或位于相同位点。EST标记GmKF059a编码一种阻遏蛋白，定位在C2组，分别占开花天数、株高、倒伏性和主茎节数总变异的约20%。
