鹰嘴豆种间杂交中生物固氮性状的标记性状关联分析（）。 注：原文括号处内容缺失，所以译文括号处也保留原样。
Marker trait association for biological nitrogen fixation traits in an interspecific cross of chickpea ().
作者信息
Nagpal Sharon, Sirari Asmita, Sharma Poonam, Singh Satinder, Mandahal Kamalpreet Singh, Singh Harpreet, Singh Sarvjeet
机构信息
Department of Microbiology, Punjab Agricultural University, Ludhiana, 141004 India.
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004 India.
出版信息
Physiol Mol Biol Plants. 2023 Jul;29(7):1005-1018. doi: 10.1007/s12298-023-01335-3. Epub 2023 Aug 19.
UNLABELLED
A set of 165 Recombinant inbred lines (RILs) derived from an interspecific cross of chickpea was used to identify QTLs for key biological nitrogen fixation (BNF) traits. The phenotyping of BNF and related traits was done at two different agroclimatic zones viz., Central plain zone (Ludhiana) and Sub-Mountainous undulating zone (Gurdaspur) for 2 consecutive seasons (2018-2020). Wild parent ILWC292 showed significantly high performance in terms of biological nitrogen fixation (BNF) traits over the cultivated GPF-2. The triple interaction of genotypes × locations × years was significant ( 0.05) for all BNF traits in parental lines. Highly significant positive correlation was obtained between grain yield and key growth and symbiotic parameters at both the sites. Phenotypic analysis revealed nodule dry weight and leghaemoglobin content as key traits for BNF efficiency and contrasting DNA bulks were constituted on the basis of these traits. Out of 535 SSR markers, 139 exhibited polymorphism between the parental lines on polyacrylamide gel electrophoresis. A total of 30 SSR markers showed polymorphism between the higher and lower bulks for nodule dry weight and leghaemoglobin content. Out of these, 20 SSRs did not show any segregation distortion in RIL population as determined by chi square analysis ( < 0.05) and were used for quantitative trait loci (QTL) analysis. Using QTL cartographer, markers- CAGM02697, CAGM09835, CAGM09777, CAGM09227, CAGM09021, CAGM08679 were found linked with QTLs for BNF. These markers can be validated further for identification of genes for BNF traits and marker assisted selection in chickpea. To the best of our knowledge this is the first report on identification of genomic regions associated with key BNF traits in chickpea across different agro-climatic zones.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12298-023-01335-3.
未标注
利用一组由鹰嘴豆种间杂交产生的165个重组自交系（RIL）来鉴定关键生物固氮（BNF）性状的数量性状基因座（QTL）。在两个不同的农业气候区，即中部平原区（卢迪亚纳）和亚山区起伏区（古尔达斯布尔）连续两个季节（2018 - 2020年）对BNF及相关性状进行了表型分析。野生亲本ILWC292在生物固氮（BNF）性状方面表现出显著高于栽培品种GPF - 2的性能。亲本系中所有BNF性状的基因型×地点×年份的三重交互作用显著（P < 0.05）。在两个地点，籽粒产量与关键生长和共生参数之间均获得了高度显著的正相关。表型分析表明，根瘤干重和豆血红蛋白含量是BNF效率的关键性状，并基于这些性状构建了对比DNA混合池。在535个SSR标记中，139个在聚丙烯酰胺凝胶电泳上显示出亲本系之间的多态性。共有30个SSR标记在根瘤干重和豆血红蛋白含量的高、低混合池之间显示出多态性。其中，20个SSR在RIL群体中经卡方分析（P < 0.05）未表现出任何分离畸变，并用于数量性状位点（QTL）分析。使用QTL Cartographer软件，发现标记CAGM02697、CAGM09835、CAGM09777、CAGM09227、CAGM09021、CAGM08679与BNF的QTL连锁。这些标记可进一步验证，以鉴定鹰嘴豆中BNF性状的基因并用于标记辅助选择。据我们所知，这是关于在不同农业气候区鉴定鹰嘴豆中与关键BNF性状相关的基因组区域的首次报道。
补充信息
在线版本包含可在10.1007/s12298 - 023 - 01335 - 3获取的补充材料。
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