Wang Zhuo, Liang Huan-Huan, Li Ben-Shang, Huang Xiao-Hang, Zhang Jing, Wang Xiang, Ding Li-Xia, Jiang Hua
Department of Hematology and Oncology, Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2013 Jul;15(7):509-13.
To study the expression of zinc finger protein X-linked (ZFX) in bone marrow mononuclear cells (BMMCs) of children with B lineage acute lymphoblastic leukemia (B-ALL) and its relationship with prognosis.
The expression of ZFX in human leukemia cell lines (REH, HL-60, NB(4) and K562) was measured by Western blot. ZFX gene was cloned by PCR from one patient and DNA sequencing technology was used to confirm it. Real-time PCR was used for detecting ZFX mRNA expression in the BMMCs of 82 children with newly-diagnosed B-ALL, 24 children with complete remission (CR) after induction therapy and 64 control children (fracture or congenital heart disease patients). According to the presence of bone marrow or central nervous system relapse during a follow-up of 3 years, the patients were identified as having a good or poor prognosis. Their ZFX mRNA levels in BMMCs at diagnosis were compared.
ZFX protein was expressed in human leukemia cell lines REH, HL-60, NB(4) and K562. ZFX mRNA expression was significantly higher in the newly-diagnosed ALL group than in the control group (P < 0.01). ZFX mRNA expression in the ALL CR group was significantly reduced compared with the newly-diagnosed ALL group (P < 0.01). Children with a poor prognosis had significantly higher ZFX mRNA levels at diagnosis than those with a good prognosis (P < 0.05).
ZFX is over-expressed in children with B-ALL and its levels are higher in those with a poor prognosis than those with a good prognosis, which suggests that ZFX is important in the prognosis evaluation of B-ALL.
研究锌指蛋白X连锁（ZFX）在B系急性淋巴细胞白血病（B-ALL）患儿骨髓单个核细胞（BMMCs）中的表达及其与预后的关系。
采用蛋白质免疫印迹法检测ZFX在人白血病细胞系（REH、HL-60、NB(4)和K562）中的表达。通过聚合酶链反应（PCR）从1例患者中克隆ZFX基因，并用DNA测序技术进行验证。采用实时荧光定量PCR检测82例新诊断的B-ALL患儿、24例诱导治疗后完全缓解（CR）患儿及64例对照儿童（骨折或先天性心脏病患者）BMMCs中ZFX mRNA的表达。根据3年随访期间是否发生骨髓或中枢神经系统复发，将患者分为预后良好或不良，比较其诊断时BMMCs中ZFX mRNA水平。
ZFX蛋白在人白血病细胞系REH、HL-60、NB(4)和K562中表达。新诊断的ALL组ZFX mRNA表达明显高于对照组（P<0.01）。ALL CR组ZFX mRNA表达较新诊断的ALL组明显降低（P<0.01）。预后不良的患儿诊断时ZFX mRNA水平明显高于预后良好的患儿（P<0.05）。
ZFX在B-ALL患儿中过度表达，预后不良者其水平高于预后良好者，提示ZFX在B-ALL预后评估中具有重要意义。
