Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA.
Nat Methods. 2013 Jun;10(6):540-7.
In the transgenic multicolor labeling strategy called ‘Brainbow’, Cre-loxP recombination is used to create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors. This method has been adapted to non-neuronal cells in mice and to neurons in fish and flies, but its full potential has yet to be realized in the mouse brain. Here we present several lines of mice that overcome limitations of the initial lines, and we report an adaptation of the method for use in adeno-associated viral vectors. We also provide technical advice about how best to image Brainbow-expressing tissue.
在被称为“Brainbow”的转基因多色标记策略中，使用 Cre-loxP 重组来在荧光蛋白之间产生随机表达选择，从而不可磨灭地用多种不同颜色标记小鼠神经元。该方法已被改编用于小鼠的非神经元细胞和鱼类及蝇类的神经元，但它在小鼠大脑中的全部潜力尚未得到实现。在此，我们介绍了几种克服初始品系限制的小鼠品系，并报告了该方法在腺相关病毒载体中的应用的一种改编。我们还提供了有关如何最佳地对表达 Brainbow 的组织进行成像的技术建议。
