Institute of Parasitic Diseases, Sichuan Centers for Disease Control and Prevention, Chengdu 610041, Sichuan, China.
Parasitology. 2013 Nov;140(13):1602-7. doi: 10.1017/S003118201300111X. Epub 2013 Jul 18.
Detection of taeniasis carriers of Taenia solium is essential for control of cysticercosis in humans and pigs. In the current study, we assessed the positive detection rate of a self-detection tool, stool microscopy with direct smear and coproPCR for taeniasis carriers in endemic Tibetan areas of northwest Sichuan. The self-detection tool through questioning about a history of proglottid expulsion within the previous one year showed an overall positive detection rate of more than 80% for Taenia saginata, T. solium and T. asiatica. The positive detection rate was similar for T. saginata and T. solium. In 132 taeniid tapeworm carriers, 68 (51·5%) were detected by microscopy and 92 (69·7%) were diagnosed by coproPCR. A combination of microscopy and coproPCR increased the positive detection rate to 77·3%. There remained 10 cases (7·6%) coproPCR negative but microscopy positive. Due to the high cost and complicated process, coproPCR is required for the identification of Taenia species only when necessary, though it had a significant higher positive detection rate than microscopy. Combined use of self-detection and stool microscopy are recommended in community-based mass screening for taeniases in this Tibetan area or in other situation-similar endemic regions.
检测带绦虫感染者对于控制人类和猪囊虫病至关重要。本研究评估了一种自检测工具（粪便直接涂片显微镜检查和粪便聚合酶链反应检测）在四川西北部藏区带绦虫病流行地区用于带绦虫感染者检测的阳性检出率。通过询问过去一年中是否有节片排出史的自检测工具对带绦虫的总体阳性检出率超过 80%，其中包括牛带绦虫、猪带绦虫和亚洲带绦虫。牛带绦虫和猪带绦虫的阳性检出率相似。在 132 例带绦虫感染者中，有 68 例（51.5%）通过显微镜检查发现，92 例（69.7%）通过 coproPCR 诊断。显微镜检查和 coproPCR 的联合使用将阳性检出率提高到 77.3%。仍有 10 例（7.6%） coproPCR 阴性但显微镜检查阳性。由于成本高且过程复杂，只有在必要时才需要通过 coproPCR 来鉴定带绦虫的种类，尽管其阳性检出率显著高于显微镜检查。建议在该藏区或其他类似流行地区的社区为基础的带绦虫病大规模筛查中，结合使用自检测和粪便显微镜检查。
