Department of Oriental Medicine Resources, Mokpo National University, Muan-gun, Jeollanam-do 534-729, South Korea; E-mail:
J Integr Med. 2013 Jul;11(4):246-52. doi: 10.3736/jintegrmed2013036.
Buckwheat has been considered as a potential source of nutraceutical components on the world market of probiotic foodstuffs. The purpose of this study was to evaluate the effects of tartary buckwheat (Fagopyrum tataricum) sprouts on oxidation and pro-inflammatory mediators.
The anti-oxidant effects of buckwheat extract (BWE) and rutin were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH)- and nitric oxide (NO)-scavenging activities, serum peroxidation and chelating assays. Lipopolysaccharide (LPS)-stimulated RAW264.7 cells were used to evaluate anti-inflammatory activities of buckwheat and rutin. NO production in LPS-stimulated RAW264.7 cells was determined by using Griess reagent. The expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB) p65 subunit in cytosolic and nuclear portions were determined by Western blot analysis. Also, the production of inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was determined by enzyme-linked immunosorbent assay.
Inhibitory concentration 50 values for DPPH- and NO-scavenging activities of BWE were 24.97 and 72.54 μg/mL respectively. BWE inhibited serum oxidation and possessed chelating activity. Furthermore, BWE inhibited IL-6 and TNF-α production in LPS-stimulated RAW264.7 cells. Also, BWE inhibited iNOS and COX-2 expression and NF-κB p65 translocation.
Buckwheat sprouts possessed strong antioxidant activity and inhibited production of pro-inflammatory mediators in the applied model systems. Thus, buckwheat can be suggested to be beneficial in inflammatory diseases by inhibiting the free radicals and inflammatory mediators.
荞麦已被视为益生菌食品世界市场中具有营养成分的潜在来源。本研究旨在评估苦荞（Fagopyrum tataricum）芽的抗氧化和促炎介质的作用。
使用 1,1-二苯基-2-苦基肼（DPPH）和一氧化氮（NO）清除活性、血清过氧化和螯合测定来评估荞麦提取物（BWE）和芦丁的抗氧化作用。使用脂多糖（LPS）刺激 RAW264.7 细胞来评估荞麦和芦丁的抗炎活性。通过使用Griess 试剂测定 LPS 刺激的 RAW264.7 细胞中的 NO 产生。通过 Western blot 分析测定诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、核因子-κB（NF-κB）p65 亚基在胞质和核部分的表达。还通过酶联免疫吸附测定法测定促炎细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的产生。
BWE 的 DPPH 和 NO 清除活性的 50 抑制浓度值分别为 24.97 和 72.54 μg/mL。BWE 抑制血清氧化并具有螯合活性。此外，BWE 抑制 LPS 刺激的 RAW264.7 细胞中 IL-6 和 TNF-α的产生。此外，BWE 抑制 iNOS 和 COX-2 表达和 NF-κB p65 易位。
荞麦芽具有很强的抗氧化活性，并抑制了应用模型系统中促炎介质的产生。因此，荞麦可以通过抑制自由基和炎症介质而有益于炎症性疾病。
