Fredman P, Månsson J E, Bigner S H, Wikstrand C J, Bigner D D, Svennerholm L
Department of Psychiatry and Neurochemistry, Gothenburg University, St. Jörgen Hospital, Hisings Backa, Sweden.
Biochim Biophys Acta. 1990 Aug 6;1045(3):239-44. doi: 10.1016/0005-2760(90)90126-i.
This study was undertaken to characterize gangliosides in the human glioma cell line U-118 MG. The cell line was grown both in cell culture and as xenografts in nude rats. A common finding in both culture and xenograft cells was the high proportion of the lactoseries ganglioside 3'-LM1, approximately one third of the total ganglioside sialic acid. Otherwise, there were marked differences between the two cell sources. The cells grown in culture had a more simple ganglioside pattern than those grown in xenografts. In the latter instance, more complex gangliosides of the lactoseries, including 3'8'-LD1, sialyllactonorhexaosylceramide and a branched structure with two terminal NeuAc alpha 2-3Gal beta 1- 4GlcNAc chains, and the gangliotetraose series were found. Another marked difference involved GM2, which in the cultured cells was a major fraction, indicating that the synthesis of the gangliotetraose series gangliosides in the former stopped at the level of GM2. These results show that the ganglioside composition of a glioma cell line is strongly influenced by environmental factors.
本研究旨在对人胶质瘤细胞系U-118 MG中的神经节苷脂进行表征。该细胞系在细胞培养中生长，并作为裸鼠异种移植物生长。在培养细胞和异种移植细胞中共同的发现是乳糖系列神经节苷脂3'-LM1的比例很高，约占总神经节苷脂唾液酸的三分之一。否则，两种细胞来源之间存在明显差异。在培养中生长的细胞比在异种移植中生长的细胞具有更简单的神经节苷脂模式。在后一种情况下，发现了乳糖系列更复杂的神经节苷脂，包括3'8'-LD1、唾液酸乳糖六糖神经酰胺和具有两条末端NeuAcα2-3Galβ1-4GlcNAc链的分支结构，以及神经节四糖系列。另一个显著差异涉及GM2，在培养细胞中它是主要成分，这表明前者中神经节四糖系列神经节苷脂的合成在GM2水平停止。这些结果表明，胶质瘤细胞系的神经节苷脂组成受到环境因素的强烈影响。
